Altered gut microbiota correlated with systemic inflammation in children with Kawasaki disease
Altered gut microbiota correlated with systemic inflammation in children with Kawasaki disease
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DOI：
10.1038/s41598-020-71371-6
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发表时间：
2020-09-03
影响因子：
4.6
通讯作者：
Cao, Youde
中科院分区：
文献类型：
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作者：
Chen, Jie;Yue, Yanghua;Cao, Youde
川崎病（Kawasaki disease， KD）是一种病因不明的多系统血管炎，主要发生于儿童，肠道菌群紊乱通常被认为是引发川崎病的超免疫反应。该研究的目的是调查粪便微生物群的变化，并评估其与全身性炎症的关系。共纳入30名KD儿童，随访6个月，另一组30名年龄和性别匹配的健康儿童作为对照。采用16S rRNA基因测序分析粪便微生物群落的种型谱。用流式细胞仪检测血清炎症标志物。我们发现，与健康对照相比，KD儿童在急性期的粪便微生物多样性显著减少。急性KD患儿肠球菌、不动杆菌、幽门螺杆菌、乳球菌、葡萄球菌和丁酸单胞菌的检出率明显高于健康儿童。急性KD患儿的全身炎症生物标志物，包括IL-2、IL-4、IL-6、IL-10、tnf - α和inf - γ水平显著升高。肠道球菌和幽门螺杆菌丰度的改变与IL-6呈正相关，这在KD中从未报道过。本研究提示肠道菌群改变与全身性炎症密切相关，为研究KD的病因和发病机制提供了新的视角。
Kawasaki disease (KD) is a multi-systemic vasculitis of unknown etiology that occurs mainly in children, and the disturbance of gut microbiota is generally believed to cause a hyperimmune reaction triggering KD. The aim of the study was to investigate the alterations in the fecal microbiota and assess its relationship with systemic inflammation. Totally 30 KD children were enrolled and followed up for 6 months, with another group of 30 age- and sex-matched healthy children as controls. Phylotype profiles of fecal microbial communities were analyzed using 16S rRNA gene sequencing. Serum inflammatory markers were detected by flow cytometer. We showed that KD children exhibited a significant reduction in fecal microbial diversity in the acute phase compared with the healthy controls. Enterococcus, Acinetobacter, Helicobacter, Lactococcus, Staphylococcus and Butyricimonas in acute KD children were significantly higher than the healthy children. Levels of systemic inflammation biomarkers, including IL-2, IL-4, IL-6, IL-10, TNF-alpha, and INF-gamma, were significantly elevated in the acute KD children. Altered microbiota genera Enterococcus and Helicobacter abundances were shown to be correlated positively with IL-6, which were never previously reported in KD. This study suggested that gut microbiota alteration is closely associated with systemic inflammation, which provides a new perspective on the etiology and pathogenesis of KD.