Differentiation of Fusarium solani f. sp. cucurbitae races 1 and 2 by random amplification of polymorphic DNA
Differentiation of Fusarium solani f. sp. cucurbitae races 1 and 2 by random amplification of polymorphic DNA
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f. 茄病镰刀菌的分化
DOI：
10.1007/bf00317067
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发表时间：
1991
期刊：
影响因子：
2.5
通讯作者：
M. Templeton
中科院分区：
文献类型：
--
作者：
R. Crowhurst;B. T. Hawthorne;E. Rikkerink;M. Templeton
我们使用了一种基于pcr的技术，包括随机扩增多态性DNA (RAPD)，来评估21株f。solanif。黄瓜品种1和2。根据RAPD标记模式，分离物可分为两个不同的群体，分别对应于交配群体MPI和MPV。通过RAPD模式对传统方法不能归属于交配群体的4个分离株进行了区分。利用7个多态RAPD产物对MPI和MPV基因组DNA进行Southern印迹检测。七个探针中有六个与单拷贝序列杂交，七个探针中有五个显示出一个或其他交配群体的特异性。我们认为，该技术不仅是一种快速可靠的真菌分离分型工具，而且还为获得物种或种族特异性杂交探针提供了一种快速方法。
We have used a PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), to assess genome variability between 21 isolates fromF. solanif. sp.cucurbitaeraces 1 and 2. Based on RAPD marker patterns the isolates fell into two distinct groups corresponding to mating populations MPI and MPV. Four isolates that could not be assigned to one or other mating population by traditional means were distinguished by RAPD patterns. Seven polymorphic RAPD products were used to probe Southern blots of MPI and MPV genomic DNA. Six of the seven probes hybridized to single-copy sequences and five of the seven probes showed specificity for one or other mating population. We suggest that not only is the technique a rapid and reliable tool for isolate-typing of fungi but it also provides a rapid method for obtaining species- or racespecific hybridization probes.
影响因子：
56.9
作者：
SAIKI, RK;SCHARF, S;ARNHEIM, N
通讯作者：
ARNHEIM, N