COMPARISON OF INFLUENZA SEROLOGICAL TECHNIQUES BY INTERNATIONAL COLLABORATIVE STUDY
COMPARISON OF INFLUENZA SEROLOGICAL TECHNIQUES BY INTERNATIONAL COLLABORATIVE STUDY
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DOI：
10.1016/0264-410x(94)90056-6
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发表时间：
1994-02-01
期刊：
影响因子：
5.5
通讯作者：
CHAKRAVERTY, P
中科院分区：
文献类型：
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作者：
WOOD, JM;GAINESDAS, RE;CHAKRAVERTY, P
进行了一项国际合作研究，以调查流感血清学技术的重现性。代表五个国家的七个实验室的参与者测量了 11 份人类血清和 3 份感染后雪貂血清中的 A/Sichuan/2/87 (H3N2)、A/Taiwan/1/86 (H1N1) 和 B/Beijing/1/87 流感病毒抗体。使用两种不同的血清学技术，血凝抑制 (HI) 和单径向溶血 (SRH)，尽管每种技术在实验室内可重复，但实验室之间的变异性对于 HI（最大变异性 32 倍；几何变异系数，GCV，112%）高于 SRH（最大变异性 3.8 倍；GCV 57%）。标准血清的使用可以直接比较 HI 和 SRH 数据，并且对于每种技术，标准血清改善了实验室间一致性。对于甲型流感病毒，HI 和 SRH 抗体之间存在相关性（相关系数类似于 0.9）。人血清中 1:40 的 HI 滴度对应于 19-33 mm(2) 的 SRH 滴度。研究结果表明，如果两种血清的 HI 滴度相差 > 4 倍且 SRH 面积相差 > 50%，则预计它们将含有不同的抗体水平。 SRH 和 HI 对甲型流感病毒抗体的检测具有相同的灵敏度，但 SRH 对乙型流感病毒抗体的检测更敏感。该研究提供了有关抗体测定标准化的宝贵信息。
An international collaborative study was performed to investigate the reproducibility of influenza serological techniques. Participants in seven laboratories representing five countries measured antibody to A/Sichuan/2/87 (H3N2), A/Taiwan/1/86 (H1N1) and B/Beijing/1/87 influenza viruses in 11 human sera and three postinfection ferret sera. Two different serological techniques were used, haemagglutination inhibition (HI) and single-radial haemolysis (SRH) and, although each technique was reproducible within laboratories, variability between laboratories was higher for HI (maximum variability 32-fold; geometric coefficient of variation, GCV, 112%) than for SRH (maximum variability 3.8-fold; GCV 57%). The use of a standard serum allowed direct comparison of HI and SRH data and, for each technique, a standard serum improved inter-laboratory agreement. For influenza A viruses there was a correlation between HI and SRH antibodies (correlation coefficient similar to 0.9). An HI titre of 1:40 in human sera corresponded to an SRH titre of 19-33 mm(2). The results of the study indicate that two sera would be expected to contain different antibody levels if their HI titres differed by >fourfold and SRH areas differed by >50%. Both SRH and HI possessed equivalent sensitivity for measurement of antibody to influenza A viruses but SRH was more sensitive for detection of antibody to influenza B viruses. The study provided valuable information about standardization of antibody assays.