Exogenous Application of GABA Improves PEG-Induced Drought Tolerance Positively Associated with GABA-Shunt, Polyamines, and Proline Metabolism in White Clover.
Exogenous Application of GABA Improves PEG-Induced Drought Tolerance Positively Associated with GABA-Shunt, Polyamines, and Proline Metabolism in White Clover.
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外源施用 GABA 可提高白三叶草 PEG 诱导的耐旱性，与 GABA 分流、多胺和脯氨酸代谢呈正相关
DOI：
10.3389/fphys.2017.01107
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发表时间：
2017
影响因子：
4
通讯作者：
Peng Y
中科院分区：
文献类型：
--
作者：
Yong B;Xie H;Li Z;Li YP;Zhang Y;Nie G;Zhang XQ;Ma X;Huang LK;Yan YH;Peng Y
为了研究外源γ-氨基丁酸对白三叶耐旱性的生理效应，研究了外源GABA(8 MM)对白三叶耐旱性的生理影响，测定了GABA的分流、多胺(PAS)和Pro的代谢。在本研究中，外源GABA有效地缓解了干旱诱导的叶片伤害，表现为显著提高叶片相对含水量，降低电解质渗漏率，降低膜脂过氧化和叶片枯萎。外源GABA进一步促进了干旱诱导的GABA转氨酶和α-酮戊二酸脱氢酶活性的升高，但抑制了谷氨酸脱羧酶的活性，导致内源谷氨酸(Glu)和GABA含量的增加。此外，外源GABA能很好地促进PAS的合成，抑制PAS的分解代谢，导致干旱胁迫下不同类型PAS含量(游离Put和Spd、不溶性结合Spd和Spm、可溶性结合Spd和Spm以及总Put、Spd和Spm)显著增加。此外，外源GABA处理进一步激活了干旱诱导的Δ1-吡咯烷-5-羧酸合成酶和Pro脱氢酶活性，但抑制了干旱促进的鸟氨酸-δ-氨基转移酶活性，导致干旱中期和末期GABA处理植株的Pro积累和代谢增加。结果表明，外源GABA处理增加内源GABA可以提高白三叶的抗旱性，并对GABA的分流、PAS和Pro代谢起正向调节作用。
In order to investigate the physiological effects of exogenous γ-aminobutyric acid (GABA) on drought tolerance in white clover (Trifolium repens), GABA shunt, polyamines (PAs), and proline (Pro) metabolism were examined after plants pretreated with or without GABA (8 mM) and then exposed to water or 15% PEG-induced drought stress in growth chamber. In this study, exogenous application of GABA effectively alleviated drought-induced damage in leaves, as reflected by significantly higher relative water content, lower electrolyte leakage, lipid peroxidation, and leaf wilt. Exogenous GABA further promoted drought-induced increases in GABA transaminase and alpha ketone glutarate dehydrogenase activities, but inhibited glutamate decarboxylase activity under both control and drought conditions, resulting in an increase in endogenous glutamate (Glu) and GABA content. Besides, exogenous GABA could well accelerated PAs synthesis and suppressed PAs catabolism, which lead to the extremely enhanced different types of PAs content (free Put and Spd, insoluble bound Spd and Spm, soluble conjugated Spd and Spm, and total Put, Spd and Spm) under drought stress. In addition, exogenous GABA application further activated drought-induced Δ1-pyrroline-5-carboxylate synthetase and proline dehydrogenase activities, but suppressed drought-facilitated ornithine -δ-amino transferase activities, leading to a higher Pro accumulation and metabolism in GABA-pretreated plants in the middle and last period of drought. The results suggested that increased endogenous GABA by exogenous GABA treatment could improve drought tolerance of white clover associated with a positive regulation in the GABA-shunt, PAs and Pro metabolism.
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影响因子：
7.4
作者：
Bhatnagar, P;Glasheen, BM;Minocha, SC
通讯作者：
Minocha, SC
影响因子：
7.4
作者：
Hong, ZL;Lakkineni, K;Verma, DPS
通讯作者：
Verma, DPS
影响因子：
1.5
作者：
Al-Quraan, N. A.;Al-Share, A. T.
通讯作者：
Al-Share, A. T.
影响因子：
6.4
作者：
Ansari, MI;Lee, RH;Chen, SCG
通讯作者：
Chen, SCG
影响因子：
6.5
作者：
Alcazar, Ruben;Planas, Joan;Altabella, Teresa
通讯作者：
Altabella, Teresa