In Vitro Exposure of Neuronal Networks to a GSM-1800 Signal
In Vitro Exposure of Neuronal Networks to a GSM-1800 Signal
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DOI：
10.1002/bem.21805
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发表时间：
2013-12-01
影响因子：
1.9
通讯作者：
Lewis, Noelle
中科院分区：
文献类型：
--
作者：
Moretti, Daniela;Garenne, Andre;Lewis, Noelle
就生物效应而言，中枢神经系统是移动电话射频（RF）场暴露最有可能的目标。一些脑电图（EEG）研究报告了在RF暴露期间和/或之后，静息脑电图和睡眠期间α波段功率谱的变化。在这种情况下，观察射频暴露下神经元网络的自发电活动可以成为检测低水平射频对神经系统影响的有效工具。我们的研究小组已经开发了一个专用的实验装置，在GHz范围内同时暴露神经网络和监测电活动。采用横向电磁（TEM）细胞将神经元网络暴露于SAR水平为3.2W/kg的GSM-1800信号下。使用微电极阵列（MEAs）记录神经元电活动并检测暴露下的细胞外尖峰和爆发。这项工作提供了关于暴露设置、神经元网络培养、电活动记录和射频暴露下获得的信号分析的综合调查的可行性和初步结果的证明。在这项对16个培养物进行的初步研究中，暴露于射频3分钟后，发射率（FR）和爆炸率（BR）可逆降低30%。需要更多的实验来进一步表征这种效应。中国生物医学工程学报，2013。(c) 2013 Wiley Periodicals, Inc.；
The central nervous system is the most likely target of mobile telephony radiofrequency (RF) field exposure in terms of biological effects. Several electroencephalography (EEG) studies have reported variations in the alpha-band power spectrum during and/or after RF exposure, in resting EEG and during sleep. In this context, the observation of the spontaneous electrical activity of neuronal networks under RF exposure can be an efficient tool to detect the occurrence of low-level RF effects on the nervous system. Our research group has developed a dedicated experimental setup in the GHz range for the simultaneous exposure of neuronal networks and monitoring of electrical activity. A transverse electromagnetic (TEM) cell was used to expose the neuronal networks to GSM-1800 signals at a SAR level of 3.2W/kg. Recording of the neuronal electrical activity and detection of the extracellular spikes and bursts under exposure were performed using microelectrode arrays (MEAs). This work provides the proof of feasibility and preliminary results of the integrated investigation regarding exposure setup, culture of the neuronal network, recording of the electrical activity, and analysis of the signals obtained under RF exposure. In this pilot study on 16 cultures, there was a 30% reversible decrease in firing rate (FR) and bursting rate (BR) during a 3min exposure to RF. Additional experiments are needed to further characterize this effect. Bioelectromagnetics 34:571-578, 2013. (c) 2013 Wiley Periodicals, Inc.