Comparison of Bacteroides-Prevotella 16S rRNA genetic markers for fecal samples from different animal species
Comparison of Bacteroides-Prevotella 16S rRNA genetic markers for fecal samples from different animal species
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DOI：
10.1128/aem.71.10.5999-6007.2005
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发表时间：
2005-10-01
影响因子：
4.4
通讯作者：
Voytek, MA
中科院分区：
文献类型：
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作者：
Fogarty, LR;Voytek, MA
为了有效地管理地表和地下沃茨，有必要提高我们检测和识别粪便污染源的能力。我们评估了使用厌氧细菌群拟杆菌-普雷沃氏菌作为潜在的粪便指示剂。来自该组的16 S rRNA基因的末端限制性长度多态性（T-RFLP）用于确定群体差异，并识别鸡、牛、鹿、狗、鹅、马、人、猪和海鸥中的任何独特群体。在除禽类以外的所有试验组中，该组似乎是一个良好的潜在粪便指示物。类杆菌-普雷沃氏菌群落T-RFLP图谱的聚类分析表明，来自同一宿主物种的样本的类杆菌-普雷沃氏菌种群比来自不同来源物种的样本更相似。我们无法鉴定出任何来源物种独有的独特峰;然而，对于大多数宿主物种，至少有一个T-RFLP峰被鉴定为在该物种中更常见，并且可以使用峰的组合来鉴定来源。从掺有已知来源粪便的水中获得的T-RFLP图谱包含来自来源的预期诊断峰。这些结果表明，鉴定与宿主种相关的类杆菌-普雷沃菌分子标记的方法可能有助于识别环境中的粪便污染源。
To effectively manage surface and ground waters it is necessary to improve our ability to detect and identify sources of fecal contamination. We evaluated the use of the anaerobic bacterial group Bacteroides-Prevotella as a potential fecal indicator. Terminal restriction length polymorphism (T-RFLP) of the 16S rRNA genes from this group was used to determine differences in populations and to identify any unique populations in chickens, cows, deer, dogs, geese, horses, humans, pigs, and seagulls. The group appears to be a good potential fecal indicator in all groups tested except for avians. Cluster analysis of Bacteroides-Prevotella community T-RFLP profiles indicates that Bacteroides-Prevotella populations from samples of the same host species are much more similar to each other than to samples from different source species. We were unable to identify unique peaks that were exclusive to any source species; however, for most host species, at least one T-RFLP peak was identified to be more commonly found in that species, and a combination of peaks could be used to identify the source. T-RFLP profiles obtained from water spiked with known-source feces contained the expected diagnostic peaks from the source. These results indicate that the approach of identifying Bacteroides-Prevotella molecular markers associated with host species might be useful in identifying sources of fecal contamination in the environment.