Label-free immunosensor based on one-step electrodeposition of chitosan-gold nanoparticles biocompatible film on Au microelectrode for determination of aflatoxin B1 in maize
Label-free immunosensor based on one-step electrodeposition of chitosan-gold nanoparticles biocompatible film on Au microelectrode for determination of aflatoxin B1 in maize
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基于金微电极上壳聚糖-金纳米颗粒生物相容膜一步电沉积的无标记免疫传感器测定玉米中黄曲霉毒素B-1
DOI：
10.1016/j.bios.2016.01.063
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发表时间：
2016-06-15
影响因子：
12.6
通讯作者：
Zhen, Tong
中科院分区：
文献类型：
--
作者：
Ma, Haihua;Sun, Jizhou;Zhen, Tong
以壳聚糖（CHI）为载体，在Au微电极上一步电沉积法制备了分散性好、粒径小（约10 nm）的金纳米粒子（AuNPs）。纳米结构的CHI-AuNPs复合膜具有丰富的胺基、良好的导电性、优异的生物相容性和抗体固定的稳定性。黄曲霉毒素B-1（AF B（1））与固定化抗体结合后，对电子传递产生了屏障，导致电流下降。采用扫描电镜（SEM）、循环伏安（CV）、电化学阻抗谱（EIS）和傅里叶变换红外光谱（FT-IR）等方法对修饰微电极的形貌和表征进行了研究。该传感器对黄曲霉毒素B1的浓度在0.1 ~ 1 ng mL（-1）和1 ~ 30 ng mL（-1）范围内具有良好的线性响应，检测限为0.06 ng mL（-1）（S/N=3）。该免疫测定法还用于分析添加黄曲霉毒素B的玉米样品（1）。考虑到样品提取程序，评估的线性范围和检测限分别为1.6-16 ng mL（-1）和0.19 ng mL（-1）。该方法简单，具有良好的可控性和重复性，可用于免疫传感器的设计。(C)2016爱思唯尔B. V.保留所有权利。
Gold nanoparticles (AuNPs) embedded in chitosan (CHI) film, well-dispersed and smaller in size (about 10 nm), were fabricated by one-step electrodeposion on Au microelectrode in solution containing chitosan and chloride trihydrate. The nano-structure CHI-AuNPs composite film offers abundant amine groups, good conductivity, excellent biocompatibility and stability for antibody immobilization. The combination of aflatoxin B-1 (AFB(1)) with immobilized antibody introduces a barrier to electron transfer, resulting in current decreasement. The morphologies and characterizations of modified microelectrodes were investigated by scanning electron microscope (SEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and Fourier transform infrared spectroscopy (FT-IR). The proposed non enzyme and label-free immunosensor exhibited high sensitive amperometric response to AFB(1) concentration in two linear ranges of 0.1 to 1 ng mL(-1) and 1 to 30 ng mL(-1), with the detection limit of 0.06 ng mL(-1) (S/N=3). The immunoassay was also applied for analysis of maize samples spiked with AFB(1). Considering the sample extraction procedure, the linear range and limit of detection were assessed to be 1.6-16 ng mL(-1) and 0.19 ng mL(-1) respectively. The simple method showed good fabrication controllability and reproducibility for immunosensor design. (C) 2016 Elsevier B.V. All rights reserved.