ROUND SPERMATID NUCLEI INJECTED INTO HAMSTER OOCYTES FORM PRONUCLEI AND PARTICIPATE IN SYNGAMY
ROUND SPERMATID NUCLEI INJECTED INTO HAMSTER OOCYTES FORM PRONUCLEI AND PARTICIPATE IN SYNGAMY
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DOI：
10.1095/biolreprod48.2.219
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发表时间：
1993-02-01
影响因子：
3.6
通讯作者：
YANAGIMACHI, R
中科院分区：
文献类型：
--
作者：
OGURA, A;YANAGIMACHI, R
圆形精细胞是刚刚完成减数分裂的生精细胞。为了发现这些单倍体细胞的细胞核是否在遗传上准备好受精，通过显微外科技术将细胞核单独注射到成熟的仓鼠卵母细胞中。在大多数成功注射并激活的卵母细胞中，精子核转化为原核并进行DNA合成。最终，在第一次卵裂之前，它们的染色体与母染色体混合在一起。虽然我们不能确定这些受精卵的发育潜力，但精子核似乎能够参与交配。
Round spermatids are spermatogenic cells that have just completed meiosis. To discover whether nuclei of these haploid cells are genetically ready for fertilization, nuclei were individually injected into mature hamster oocytes via a microsurgical technique. In the majority of oocytes that were successfully injected and activated, spermatid nuclei transformed into pronuclei and underwent DNA synthesis. Ultimately their chromosomes mingled with ootid chromosomes immediately prior to the first cleavage. Although we could not determine the developmental potential of these zygotes, spermatid nuclei appear capable of participating in syngamy.