Relationship between vascular damage degrees and endothelial progenitor cells in patients with erectile dysfunction: Effect of vardenafil administration and PDE5 expression in the bone marrow
Relationship between vascular damage degrees and endothelial progenitor cells in patients with erectile dysfunction: Effect of vardenafil administration and PDE5 expression in the bone marrow
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DOI：
10.1016/j.eururo.2006.08.052
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发表时间：
2007-05-01
期刊：
影响因子：
23.4
通讯作者：
Ferlin, Alberto
中科院分区：
文献类型：
--
作者：
Foresta, Carlo;Caretta, Nicola;Ferlin, Alberto
目的：评价勃起功能障碍（艾德）和颈动脉水平不同程度血管损伤的男性患者循环祖细胞（PC）水平以及单剂量伐地那非20 mg对这些细胞数量的影响。方法：选择艾德伴不同程度颈动脉损伤患者68例，对照组25例。根据患者的内膜中层厚度（IMT）状态（正常、轻度增加或斑块）将患者分为三组。所有受试者均接受伐地那非20 mg，并在基线和伐地那非给药后4 h评价循环PC数量。还进行了骨髓上磷酸二酯酶5型（PDES）的RNA表达分析。结果如下：我们发现，与对照组相比，艾德患者的循环PC显著减少，轻度IMT增加或斑块患者的PC计数减少，但IMT正常的患者则没有。伐地那非给药后4小时，我们观察到所有患者和对照组中PC数量增加。逆转录-聚合酶链反应分析表明，人骨髓表达PDE 5信使RNA。结论：患有艾德和循环PC数量低的患者可能被认为是内皮功能障碍的风险增加。对伐地那非刺激的反应受损可被提议作为患者内皮再生能力的替代标记。(C)2006年欧洲泌尿外科协会。Elsevier B. V.出版，保留所有权利。
Objectives: To evaluate the levels of circulating progenitor cells (PCs) and the effect of a single dose of vardenafil 20 mg on the number of these cells in men with erectile dysfunction (ED) and various degree of vascular injury at the carotid artery level. Methods: Sixty-eight patients with ED and various degree of carotid damage, and 25 controls were enrolled. Patients were divided into three groups according to their intima media thickness (IMT) status (normal, mild increase, or plaque). All subjects received vardenafil 20 mg, and evaluation of the number of circulating PCs was performed at baseline and 4 h after vardenafil administration. An RNA expression analysis of phosphodiesterase type 5 (PDES) on bone marrow was also performed. Results: We found a significant reduction of circulating PCs in ED patients with respect to controls and a reduction in PC counts in patients with mild IMT increase or plaque, but not in those with normal IMT. Four hours after vardenafil administration we observed an increase in the number of PCs in all patients and controls. Reverse transcriptase-polymerase chain reaction analysis showed that human bone marrow expresses PDE5 messenger RNA. Conclusions: Patients with ED and a low number of circulating PCs may be considered at increased risk for an endothelial dysfunction. An impaired response to vardenafil stimulus may be proposed as a surrogate marker of a patient's endothelial regenerative ability. (C) 2006 European Association of Urology. Published by Elsevier B.V. All rights reserved.