Bursal and postbursal stem cells in chicken. functional characteristics
Bursal and postbursal stem cells in chicken. functional characteristics
复制标题
鸡的法氏囊和法氏囊后干细胞。
DOI：
--
复制
发表时间：
1973
影响因子：
5.4
通讯作者：
A. Toivanen
中科院分区：
文献类型：
--
作者：
P. Toivanen;A. Toivanen
用环磷酰胺(CY)处理或手术切除法氏囊，用CY处理3日龄雏鸡，注射来自不同年龄供者的法氏囊或骨髓细胞。细胞接受者和供者在主要组织相容性基因座上是同源的。细胞移植后5~6周，检测小鼠对绵羊红细胞和流产布鲁氏菌的抗体反应，以及脾和法氏囊的显微形态。法氏囊的相对重量被发现是法氏囊结构恢复的可靠指标。结果表明，鸡B细胞系的干细胞或祖细胞可分为法氏囊干细胞和法氏囊后干细胞。这两种细胞类型都能有效地恢复环磷酰胺处理的鸡的抗体形成。
Cyclophosphamide (CY)‐treated or surgically bursectomized, CY‐treated 3‐day old chicks were injected with bursa or bone marrow cells from donors of different ages. Cell recipients and donors were isogeneic at the major histocompatibility locus. Antibody responses to sheep red blood cells and Brucella abortus, and microscopic morphology of spleen and bursa were assessed 5–6 weeks after the cell transplantation. Relative weight of the bursa was found to be a reliable indicator of the restoration of bursal structure. The results indicate that stem cells or progenitor cells for the B cell line in chicken can be divided into a bursal stem cell and a postbursal stem cell. Both of these cell types are effective in restoring antibody formation of CY‐treated chickens.