SIGNALING OF TRICHOMONAS-VAGINALIS FOR AMEBOID TRANSFORMATION AND ADHESIN SYNTHESIS FOLLOWS CYTOADHERENCE
SIGNALING OF TRICHOMONAS-VAGINALIS FOR AMEBOID TRANSFORMATION AND ADHESIN SYNTHESIS FOLLOWS CYTOADHERENCE
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DOI：
10.1111/j.1365-2958.1993.tb01121.x
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发表时间：
1993-01-01
影响因子：
3.6
通讯作者：
ALDERETE, JF
中科院分区：
文献类型：
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作者：
ARROYO, R;GONZALEZROBLES, A;ALDERETE, JF
阴道毛滴虫是一种性传播的鞭毛原虫，其与阴道上皮细胞的粘附是其感染的关键。电子显微镜观察发现，在体外生长的寄生虫具有典型的球形形状转化后迅速与血管内皮细胞接触成薄，扁平，变形虫细胞，最大限度地增加粘附到血管内皮细胞表面的面积。阿米巴毛滴虫形成丝状伪足和伪足，它们在靶细胞质膜上的不同位点相互交叉。而T.与HeLa细胞（先前使用的体外宿主模型细胞）相互作用的迷走神经。最初寄生的VEC由一个单一的有机体，其次是建立一个单层的毛滴虫的宿主细胞。最后，寄生虫粘附到VEC或HeLa细胞被诱导合成更大量的四个先前描述的粘附素。因此，与任一上皮细胞类型接触后的不同信号导致T.流浪汉
The cytoadherence of Trichomonas vaginalis, the sexually transmitted flagellated protozoan, to vaginal epithelial cells (VECs) is the key to infection. Electron microscopy revealed that in vitro-grown parasites having typical globular shape transformed rapidly after contact with VECs into thin, flat, amoeboid cells, maximizing the area of adhesion to the surface of VECs. Amoebic trichomonads formed filopodia and pseudopodia, which interdigitated at distinct sites on the plasma membrane of target cells. In contrast, the amoeboid transformation did not occur for T. vaginalis interacting with HeLa cells, the previously used in vitro host model cell. Initial parasitism of VECs by a single organism was followed by establishment of a monolayer of trichomonads on the host cell. Finally, parasites adhering to either VECs or HeLa cells were induced to synthesize greater amounts of the four previously described adhesins. Therefore, distinct signals after contact with either epithelial cell type leads to the morphological transformation and/or induction of adhesin synthesis by T. vaginalis.