The interaction of the soft rot bacterium Pseudomonas gladioli pv agaricicola with Japanese cultivated mushrooms
The interaction of the soft rot bacterium Pseudomonas gladioli pv agaricicola with Japanese cultivated mushrooms
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DOI：
10.1139/m97-091
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发表时间：
1997-07-01
影响因子：
2.8
通讯作者：
Tsuneda, A
中科院分区：
文献类型：
--
作者：
Gill, WM;Tsuneda, A
食用菌软腐细菌假单胞菌唐氏假单胞菌木耳被观察到在接种到几种重要的日本栽培蘑菇的组织上时会引起凹陷。扫描电子显微镜研究表明，菌丝壁层被顺序移除，从而暴露出甲壳素骨架基质，而甲壳素骨架基质又被降解。第二种类型的损伤也被观察到，典型的损伤是菌丝细胞塌陷、萎缩，在某些情况下是溶解的。培养板检测结果表明，假单胞菌属于唐古氏假单胞菌。木耳产生几丁质酶，再加上早期的β-葡聚糖酶证据，解释了病原体的降解能力。黄伞孢子果表面的胶状涂层似乎具有对黄伞假单胞菌的抗性。黑木耳攻击。培养皿与几种栽培食用菌的共培养表明，假单胞菌具有较强的致病能力。木耳在很远的距离内抑制菌丝生长，并暗示存在一种或多种毒素。由于其寄主范围广，所以其寄主范围较广。木耳被认为是一个潜在的威胁，不仅对日本的蘑菇产业，而且对其他热带/亚热带国家的蘑菇产业也是如此。
The mushroom soft rot bacterium Pseudomonas gladioli pv. agaricicola was observed to cause pitting when inoculated onto tissues of several commercially important Japanese cultivated mushrooms. Scanning electron microscope studies demonstrated the sequential removal of hyphal wall layers, thereby exposing the chitin skeletal matrix, which in turn was degraded. A second type of damage typified by collapsed, shriveled, and in some cases lysed hyphal cells was also observed. Culture plate assays revealed that Pseudomonas gladioli pv. agaricicola produces chitinase and this, coupled with earlier evidence of a beta-glucanase enzyme, accounted for the degradative ability of the pathogen. The gelatinous coating on the Pholiota nameko sporocarp appeared to confer resistance to Pseudomonas gladioli pv. agaricicola attack. Petri dish coincubations with several cultivated mushroom species indicated the ability of Pseudomonas gladioli pv. agaricicola to inhibit mycelial growth over a large distance and suggested the presence of a toxin or toxins. Owing to its wide host range, Pseudomonas gladioli pv. agaricicola is considered as a potential threat, not only to the mushroom industry in Japan but also to the mushroom industry in other tropical/subtropical countries.