A comparative evaluation of accelerated solvent extraction and Polytron extraction for quantification of lipids and extractable organochlorine in fish.
A comparative evaluation of accelerated solvent extraction and Polytron extraction for quantification of lipids and extractable organochlorine in fish.
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DOI：
10.1016/j.chemosphere.2003.08.024
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发表时间：
2004
期刊：
影响因子：
8.8
通讯作者：
Wenshan Zhuang;B. Mckague;D. Reeve;J. Carey
中科院分区：
文献类型：
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作者：
Wenshan Zhuang;B. Mckague;D. Reeve;J. Carey
建立了使用加速溶剂萃取器 (ASE) 和 Polytron 均化器的萃取方法的最佳条件，用于定量鱼中的（粗）脂质和可萃取有机氯 (EOCl)。这两种方法，即在 55 和 100 °C 下对磨碎的冻干鱼片进行 ASE 连续提取，以及在室温下对磨碎的湿鱼片进行四次重复的 Polytron 提取以及对湿鱼肝进行两次重复的提取，从重现性和可比性方面进行了评估。在脂质测量方面，这两种方法具有相当的重现性，并且与采样点内鱼与鱼的自然变化相比，实验误差相对较小。对于 EOCl 测量，相对标准偏差往往会随着样品中 EOCl 水平的降低而增加。鱼基质中痕量分析物测量结果的相对标准偏差的一个重要因素是等分试样的采样误差。使用配对比较设计的统计分析表明，从这两种提取方法获得的测量值（粗脂质或EOCl）通常并不相等。两种血脂测量方法之间具有良好的相关性；而对于 EOCl 测量，暴露鱼组内存在高度相关性，但参考组中不存在。
Optimal conditions for extraction methods using an accelerated solvent extractor (ASE) and a Polytron homogenizer were established for quantification of (crude) lipids and extractable organochlorine (EOCl) in fish. The two methods, an ASE consecutive extraction at 55 and 100 °C for ground, freeze-dried filets, and a Polytron extraction at room temperature with four repetitions for ground, wet filets and with two repetitions for wet fish livers were evaluated in terms of reproducibility and comparability. With respect to lipid measurement, the two methods have comparable reproducibility and the experimental errors are relatively small compared to natural variations of fish to fish within a sampling site. For EOCl measurement, the relative standard deviation tends to increase with decreasing levels of EOCl in specimens. An important factor for the relative standard deviation of measurements of analytes present at trace levels in fish matrices is sampling error in taking aliquots. Statistical analysis using a paired comparison design showed that measurement values (crude lipids or EOCl) obtained from these two extraction methods are, in general, not equivalent. There is a good correlation between the two methods for lipid measurement; whereas for EOCl measurements, high degrees of correlation exist within the exposed fish group, but not in the reference group.