Circadian clock-controlled genes isolated from Neurospora crassa are late night- to early morning-specific
Circadian clock-controlled genes isolated from Neurospora crassa are late night- to early morning-specific
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DOI：
10.1073/pnas.93.23.13096
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发表时间：
1996-11-12
影响因子：
11.1
通讯作者：
Dunlap, JC
中科院分区：
文献类型：
--
作者：
BellPedersen, D;Shinohara, ML;Dunlap, JC
在大多数生物体中，内源生物钟控制着生命的时间方面，包括对时钟输出途径中涉及的基因的节律控制。在真菌粗糙脉孢菌中，已知的一种受生物钟控制的途径是无性孢子(分生孢子)发育。为了更全面地了解由粗毛夜蛾生物钟调节的过程，人们对在转录水平上振荡的基因进行了系统的筛选。构建了特定时间的cDNA文库，并用于差异筛选，以鉴定6个新的时钟控制基因(CCGs)。每个CCG的特定转录本优先在深夜到清晨积累，尽管它们在稳定状态下的mRNA水平和节律的幅度方面有所不同。对新的CCG cDNAs末端的测序表明，CCG-12与编码铜金属硫蛋白的粗面拟青霉CMT完全相同，这表明并不是所有粗面拟青霉的时钟调控基因都与分生孢子的发育有关，这一点得到了包括CMT(CCG-12)在内的一半新CCG的发现支持，这些新的CCG不是由发育或光信号诱导的转录。这些数据表明，时钟在调节不同于发育的生物过程中发挥着重要作用。
An endogenous circadian biological clock controls the temporal aspects of life in most organisms, including rhythmic control of genes involved in clock output pathways. In the fungus Neurospora crassa, one pathway known to be under control of the clock is asexual spore (conidia) development. To understand more fully the processes that are regulated by the N. crassa circadian clock, systematic screens were carried out for genes that oscillate at the transcriptional level. Time-of-day-specific cDNA libraries were generated and used in differential screens to identify six new clock-controlled genes (ccgs). Transcripts specific for each of the ccgs preferentially accumulate during the late night to early morning, although they vary with respect to steady-state mRNA levels and amplitude of the rhythm. Sequencing of the ends of the new ccg cDNAs revealed that ccg-12 is identical to N. crassa cmt encoding copper metallothionein, providing the suggestion that not all clock-regulated genes in N. crassa are specifically involved in the development of conidia, This was supported by finding that half of the new ccgs, including cmt (ccg-12), are not transcriptionally induced by developmental or light signals. These data suggest a major role for the clock in the regulation of biological processes distinct from development.