Antishigellosis and Cytotoxic Potency of Crude Extracts and Isolated Constituents from Duranta repens.
Antishigellosis and Cytotoxic Potency of Crude Extracts and Isolated Constituents from Duranta repens.
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抗虫病和来自Duranta repens的粗提取物和分离成分的抗细胞毒性。
DOI：
10.4489/myco.2008.36.3.173
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发表时间：
2008-09
期刊：
影响因子：
1.9
通讯作者：
Haque ME
中科院分区：
文献类型：
--
作者：
Nikkon F;Habib MR;Karim MR;Hossain MS;Mosaddik MA;Haque ME
用纸片扩散法研究了杜鹃花茎的乙醇粗提物及其提取物和两个三萜类化合物β-Amyrin和12-齐墩果烯3-β，21-β-二醇的混合物，用纸片扩散法评价了它们的抗菌、抗真菌活性，并用卤虾致死率测定了它们的细胞毒性。两个化合物的结构经红外光谱、核磁共振氢谱、核磁共振碳谱和液质联用确证。茎的氯仿可溶部分和水果的乙醇提取物具有较强的抗志贺氏菌活性，对一些病原菌和真菌也有中等的抑制活性，但分离的化合物1(β-Amyrin和12-齐墩果烯3-β，21-β-diol的混合物)对微生物生长有轻度到中度的抑制活性。茎、果提取物及其提取物和化合物1的最小抑菌浓度(MIC)为32~128µg/ml，茎的氯仿可溶部位和果实的乙醇提取物对卤虫有明显的细胞毒性，其LC_(50)值分别为0.94和0.49µg/ml。
The crude ethanol extracts (stem and fruits), their fractions and two triterpenes, β-Amyrin and 12-Oleanene 3β, 21β-diol, isolated as a mixture from the chloroform soluble fraction of an ethanolic extract of Duranta repens stem, were evaluated for antibacterial, antifungal activities by the disc diffusion method and cytotoxicity by brine shrimp lethality bioassay. The structures of the two compounds were confirmed by IR, 1H-NMR, 13C-NMR and LC-MS spectral data. The chloroform soluble fraction of stem and ethanol extract of fruits possess potent antishigellosis activity and also exhibited moderate activity against some pathogenic bacteria and fungi but the isolated compound 1 (mixture of β-Amyrin and 12-Oleanene 3β, 21β-diol) showed mild to moderate inhibitory activity to microbial growth. The minimum inhibitory concentrations (MICs) of the extracts (stem and fruits), their fractions and compound 1 were found to be in the range of 32~128 µg/ml. The chloroform soluble fractions of stem and ethanol extract of fruit showed significant cytotoxicity with LC50 value of 0.94 µg/ml and 0.49 µg/ml, respectively against brine shrimp larvae.