Rapid adhesion of Stagonospora nodorum spores to a hydrophobic surface requires pre-formed cell surface glycoproteins
Rapid adhesion of Stagonospora nodorum spores to a hydrophobic surface requires pre-formed cell surface glycoproteins
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DOI：
10.1016/j.mycres.2007.09.007
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发表时间：
2007-11-01
影响因子：
--
通讯作者：
Green, Jonathan R.
中科院分区：
文献类型：
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作者：
Newey, Lisa J.;Caten, Chris E.;Green, Jonathan R.
真菌病原菌在叶片表面的黏附是侵染过程中重要的第一步。早先对小麦和其他谷物的主要坏死性病原菌Stagospora nodorum的研究表明，分生孢子迅速附着在疏水表面，随后活跃地分泌细胞外基质物质以巩固黏附。本文研究了预制的孢子表面糖蛋白在诺道氏链霉菌分生孢子快速附着到人造表面聚苯乙烯中的作用。利用十二烷基硫酸钠(SDS)、几丁质酶和裂解酶从孢子中释放细胞壁糖蛋白，并用SDS-PAGE和Western blotting对其进行了鉴定。用荧光标记的凝集素标记也被用于研究孢子表面。结果表明，孢子壁中含有少量非共价和共价结合的糖蛋白，结构不均一。在黏附试验中，已经使用了蛋白酶、凝集素和其他处理孢子的效果，以表明预制糖蛋白与疏水表面的快速黏附有关。也有证据表明，孢子迅速释放糖蛋白，这也与黏附有关，这不是一个活跃的过程。(C)2007年英国真菌学会。爱思唯尔有限公司出版。保留所有权利。
Adhesion of fungal pathogens to leaf surfaces is an important first step in the infection process. Previous work on Stagonospora nodorum, a major necrotrophic pathogen of wheat and other cereals, has shown that conidia attach rapidly to a hydrophobic surface and this is followed by the active secretion of extracellular matrix material to consolidate adhesion. In this paper the role of pre-formed spore surface glycoproteins in the rapid adhesion of S. nodorum conidia to an artificial surface, polystyrene, has been investigated. Sodium dodecyl sulphate (SDS) and the enzymes chitinase and lyticase have been used to release cell wall glycoproteins from spores and these have been identified using SDS polyacrylamide gel electrophoresis (PAGE) and Western blotting. Labelling with fluorescently tagged lectins has also been used to study the spore surface. The results show that there are a small number of glycoproteins non-covalently and covalently attached to other components in the spore wall, which is not a uniform structure. The effects of proteases, lectins, and other treatments of spores in an adhesion assay have been used to show that preformed glycoproteins are involved in rapid adhesion to a hydrophobic surface. There is also evidence for a rapid release of glycoproteins by spores that is also involved in adhesion and this is not an active process. (C) 2007 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.