Nuclear domain 10 of the viral aspect.
Nuclear domain 10 of the viral aspect.
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DOI：
10.5501/wjv.v2.i3.110
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发表时间：
2013-08
期刊：
影响因子：
--
通讯作者：
Yisel Rivera-Molina;F. Martínez;Qiyi Tang
中科院分区：
文献类型：
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作者：
Yisel Rivera-Molina;F. Martínez;Qiyi Tang
核结构域 10 (ND10) 是分布在整个核质中的球形体，尺寸约为 0.2-1.0 μm。它们最初是在电子显微镜下观察到的，最初被描述为在原子核中发现的致密体。它们还有许多其他名称，包括早幼粒细胞白血病小体（PML 小体）、克雷默小体和 PML 致癌结构域。 ND10 经常与卡哈尔体和裂解体相关。有人认为它们在调节基因转录中发挥作用。 ND10 最初是使用人自身抗血清进行表征的，该血清可识别原发性胆汁性肝硬化患者的 100 kDa 斑点蛋白。在免疫组织化学水平上，ND10 显示为核点状结构，其中 10 表示观察到的每个核点的大致数量。 ND10 不与着丝粒、着丝粒、mRNA 加工位点或染色体共定位。 ND10 抗原对核酸酶消化和盐提取的抗性表明 ND10 与核基质相关。它们通常通过使用针对 PML、死亡域相关蛋白、核点蛋白 (NDP55) 等的特异性抗体进行免疫荧光测定来识别。 ND10 的作用长期以来一直是研究的主题，近 20 年来，ND10 与病毒感染的具体联系一直是人们特别关注的焦点。本综述总结了ND10与病毒感染的关系。还讨论了一些未来的研究方向。
Nuclear domain 10 (ND10) are spherical bodies distributed throughout the nucleoplasm and measuring around 0.2-1.0 μm. First observed under an electron microscope, they were originally described as dense bodies found in the nucleus. They are known by a number of other names, including Promyelocytic Leukemia bodies (PML bodies), Kremer bodies, and PML oncogenic domains. ND10 are frequently associated with Cajal bodies and cleavage bodies. It has been suggested that they play a role in regulating gene transcription. ND10 were originally characterized using human autoantisera, which recognizes Speckled Protein of 100 kDa, from patients with primary biliary cirrhosis. At the immunohistochemical level, ND10 appear as nuclear punctate structures, with 10 indicating the approximate number of dots per nucleus observed. ND10 do not colocalize with kinetochores, centromeres, sites of mRNA processing, or chromosomes. Resistance of ND10 antigens to nuclease digestion and salt extraction suggest that ND10 are associated with the nuclear matrix. They are often identified by immunofluorescent assay using specific antibodies against PML, Death domain-associated protein, nuclear dot protein (NDP55), and so on. The role of ND10 has long been the subject of investigation, with the specific connection of ND10 and viral infection having been a particular focus for almost 20 years. This review summarizes the relationship of ND10 and viral infection. Some future study directions are also discussed.