Enhanced cellular activation with single walled carbon nanotube bundles presenting antibody stimuli
Enhanced cellular activation with single walled carbon nanotube bundles presenting antibody stimuli
复制标题
DOI：
10.1021/nl080332i
复制
发表时间：
2008-07-01
期刊：
影响因子：
10.8
通讯作者：
Fahmy, Tarek M.
中科院分区：
文献类型：
--
作者：
Fadel, Tarek R.;Steenblock, Erin R.;Fahmy, Tarek M.
通过使用呈现抗体刺激的单壁碳纳米管 (SWNT) 束在体外扩增 T 细胞，可以实现有效的免疫治疗。由于这些束的表面积很大（可以达到 1560 m(2)/g），T 细胞刺激抗体（例如抗 CD3）可以以高局部浓度呈现，从而诱导 T 细胞的有效激活。我们发现，吸附在 SWNT 束上的抗 CD3 比同等浓度的可溶性抗 CD3 更有效地刺激细胞。吸附在 SWNT 上的抗体的刺激明显高于其他高表面积材料（活性炭、聚苯乙烯和 C60 纳米颗粒），这表明 SWNT 束具有独特的刺激呈现特性。我们通过化学处理证明了这些束的表面积可调性及其对抗体吸附和随后 T 细胞激活的影响。此外，T细胞反应随SWNT浓度的变化而呈浓度依赖性。吸附在单壁碳纳米管束上的抗体刺激代表了有效激活淋巴细胞的新范例，可用于基础科学应用和临床免疫治疗。
Efficient immunotherapy can be accomplished by expanding T cells outside the body using single walled carbon nanotube (SWNT) bundles presenting antibody stimuli. Owing to the large surface area of these bundles, which can reach 1560 m(2)/g, T cell stimulating antibodies such as anti-CD3, can be presented at high local concentrations inducing potent activation of T cells. We show that anti-CD3 adsorbed onto SWNT bundles stimulate cells more effectively than equivalent concentrations of soluble anti-CD3. Stimulation by antibody adsorbed onto SWNT is significantly higher than other high surface area materials (activated carbon, polystyrene, and C60 nanoparticles), suggesting unique properties of SWNT bundles for stimuli presentation. We demonstrate the surface area tunability of these bundles by chemical treatment and its effect on antibody adsorption and subsequent T cell activation. In addition, the T cell response varied with the concentration of SWNT in a concentration dependent manner. Antibody stimuli adsorbed onto SWNT bundles represent a novel paradigm for efficient activation of lymphocytes, useful for basic science applications and clinical immunotherapy.