Recognition of floral homeotic MADS domain transcription factors by a phytoplasmal effector, phyllogen, induces phyllody.
Recognition of floral homeotic MADS domain transcription factors by a phytoplasmal effector, phyllogen, induces phyllody.
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DOI：
10.1111/tpj.12495
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发表时间：
2014-05
期刊：
影响因子：
--
通讯作者：
Namba S
中科院分区：
文献类型：
--
作者：
Maejima K;Iwai R;Himeno M;Komatsu K;Kitazawa Y;Fujita N;Ishikawa K;Fukuoka M;Minato N;Yamaji Y;Oshima K;Namba S
植物病原体改变植物的发育过程，导致被感染的寄主植物形态异常。植物原体是一种独特的植物致病菌，它能将植物的花器官转化为叶状结构，并产生次生花。多年来，这些独特的症状引起了相当大的兴趣。在这里，我们通过关注植物原体分泌蛋白PHYL1揭示了花症状的分子机制，该蛋白诱导花的形态变化与植物原体感染植物的形态变化相似。PHYL1是植物原质效应物SAP54的同源物，也能改变花的发育。通过酵母双杂交和植物瞬时共表达实验，我们发现PHYL1与花同源MADS结构域蛋白SEPALLATA3 （SEP3）、APETALA1 （AP1）和CAULIFLOWER （CAL）相互作用并降解。MADS结构域蛋白的降解依赖于泛素-蛋白酶体途径。在35S::PHYL1转基因植株中，SEP3和AP1下游的花发育基因表达被破坏。在其他植物原体株和种中，PHYL1在遗传和功能上是保守的。我们将PHYL1、SAP54及其同源物命名为“phyllogens”基因家族的成员。
Plant pathogens alter the course of plant developmental processes, resulting in abnormal morphology in infected host plants. Phytoplasmas are unique plant-pathogenic bacteria that transform plant floral organs into leaf-like structures and cause the emergence of secondary flowers. These distinctive symptoms have attracted considerable interest for many years. Here, we revealed the molecular mechanisms of the floral symptoms by focusing on a phytoplasma-secreted protein, PHYL1, which induces morphological changes in flowers that are similar to those seen in phytoplasma-infected plants. PHYL1 is a homolog of the phytoplasmal effector SAP54 that also alters floral development. Using yeast two-hybrid and in planta transient co-expression assays, we found that PHYL1 interacts with and degrades the floral homeotic MADS domain proteins SEPALLATA3 (SEP3), APETALA1 (AP1) and CAULIFLOWER (CAL). This degradation of MADS domain proteins was dependent on the ubiquitin–proteasome pathway. The expression of floral development genes downstream of SEP3 and AP1 was disrupted in 35S::PHYL1 transgenic plants. PHYL1 was genetically and functionally conserved among other phytoplasma strains and species. We designate PHYL1, SAP54 and their homologs as members of the phyllody-inducing gene family of ‘phyllogens’.
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影响因子：
11.6
作者：
Ikeda, Miho;Mitsuda, Nobutaka;Ohme-Takagi, Masaru
通讯作者：
Ohme-Takagi, Masaru
影响因子：
7.4
作者：
Immink, Richard G. H.;Pose, David;Angenent, Gerco C.
通讯作者：
Angenent, Gerco C.
影响因子：
11.6
作者：
Gillespie, T;Boevink, P;Oparka, KJ
通讯作者：
Oparka, KJ
影响因子：
11.6
作者：
IRISH, VF;SUSSEX, IM
通讯作者：
SUSSEX, IM
影响因子：
2.7
作者：
Cettul, Emanuele;Firrao, Giuseppe
通讯作者：
Firrao, Giuseppe