Differential regulation of laccase gene expression in Coriolopsis rigida LPSC No. 232
Differential regulation of laccase gene expression in Coriolopsis rigida LPSC No. 232
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DOI：
10.1016/j.funbio.2010.09.010
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发表时间：
2010-11-01
期刊：
影响因子：
2.5
通讯作者：
Jurado, Miguel
中科院分区：
文献类型：
--
作者：
Saparrat, Mario;Balatti, Pedro A.;Jurado, Miguel
克隆了白腐菌corolopsis rigida的两个漆酶同工酶基因lcc2和lcc3，并利用定量RT-PCR分析了lcc1和lcc2的转录水平，研究了它们在一系列可能的诱变剂（Cu2+、Mn2+、Fe3+、2,6-二甲氧基-1,4-苯醌、H2O2、咖啡因、两性霉素B和丁香酸）下的表达模式。在铜的存在下，lcc1的诱导率最高，因此，通过动力学研究分析了铜对lcc1基因临时表达的影响。结果表明，铜的上调与生长阶段有关，在营养期最高，在特发性生长阶段降低。两性霉素B上调lcc1和lcc2转录本水平，丁香酸上调lcc1和lcc3转录本水平，2,6-二甲氧基-1,4-苯醌诱导lcc2和lcc3转录本表达水平。讨论了僵菌漆酶基因在转录水平上受到差异调控的可能原因。(C) 2010英国真菌学学会。Elsevier Ltd.出版。版权所有。
Two laccase isoenzyme genes (lcc2 and lcc3) from the white-rot fungus Coriolopsis rigida were cloned, and together with the previously described lcc1, their transcript levels were analysed by Quantitative RT-PCR in order to study their expression patterns under a range of putative inducers (Cu2+, Mn2+, Fe3+, 2,6-dimethoxy-1,4-benzoquinone, H2O2, caffeine, amphotericin B and syringic acid). The highest induction was observed for lcc1 in presence of copper, and thus, a kinetic study was performed to analyze its effect on temporary lcc1 gene expression. Our results showed that upregulation due to copper was linked to growth stage, being highest during the trophophase and decreasing during the idiophase. Amphotericin B increased levels of transcripts of lcc1 and lcc2, syringic acid upregulated lcc1 and lcc3 and 2,6-dimethoxy-1,4-benzoquinone induced lcc2 and lcc3. Possible reasons for why laccase genes from C. rigida are differentially regulated at the transcriptional level are discussed. (C) 2010 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.