Src-tyrosine kinases are major agents in mitochondrial tyrosine phosphorylation
Src-tyrosine kinases are major agents in mitochondrial tyrosine phosphorylation
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DOI：
10.1002/jcb.21670
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发表时间：
2008-06-01
影响因子：
4
通讯作者：
Toninello, Antonio
中科院分区：
文献类型：
--
作者：
Tibaldi, Elena;Brunati, Anna Maria;Toninello, Antonio
线粒体酪氨酸磷酸化是调节线粒体功能的重要机制。这篇文章，旨在确定哪些激酶是线粒体酪氨酸磷酸化的主要代理商，表明这一作用应归因于Src家族成员。事实上，该家族的各种成员，例如Fgr、Fyn、林恩、c-Src，组成性地存在于线粒体的内部结构中，以及Csk，其是调节该家族活性的关键酶。通过不同的方法，生化分馏，蛋白质印迹和免疫金分析“原位”的磷酸酪氨酸信号，证据是存在的信号转导途径，从质膜到线粒体，导致增加Src依赖的线粒体酪氨酸磷酸化。Src激酶在线粒体水平上的激活与增殖状态相关，其中几种线粒体蛋白质被特异性酪氨酸磷酸化。
Mitochondrial tyrosine phosphorylation is emerging as an important mechanism in regulating mitochondrial function. This article, aimed at identifying which kinases are the major agents in mitochondrial tyrosine phosphorylation, shows that this role should be attributed to Src family members. Indeed, various members of this family, for example, Fgr, Fyn, Lyn, c-Src, are constitutively present in the internal structure of mitochondria as well as Csk, a key enzyme in the regulation of the activity of this family. By means of different approaches, biochemical fractioning, Western blotting and immunogold analysis "in Situ" of phosphotyrosine signaling, evidence is reported on the existence of a signal transduction pathway from plasma membrane to mitochondria, resulting in increasing Src-dependent mitochondrial tyrosine phosphorylation. The activation of Src kinases at mitochondrial level is associated with the proliferative status where several mitochondrial proteins are specifically tyrosine-phosphorylated.