Identification and in silico characterization of soybean trihelix-GT and bHLH transcription factors involved in stress responses.
Identification and in silico characterization of soybean trihelix-GT and bHLH transcription factors involved in stress responses.
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DOI：
10.1590/s1415-47572012000200005
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发表时间：
2012-06
影响因子：
2.1
通讯作者：
Margis-Pinheiro M
中科院分区：
文献类型：
--
作者：
Osorio MB;Bücker-Neto L;Castilhos G;Turchetto-Zolet AC;Wiebke-Strohm B;Bodanese-Zanettini MH;Margis-Pinheiro M
非生物或生物因素引起的环境胁迫对农业影响很大。大豆[Glycine max（L.）作为世界上最重要的作物品种之一，情况也不例外。为了应对这些逆境，植物进化出了多种复杂的分子机制，其中转录因子对靶基因的转录调控是至关重要的。尽管许多转录因子家族在逆境应答中的作用已被广泛报道，但仍有许多问题有待研究，特别是在大豆中。因此，本研究的目的是研究bHLH和trihelix-GT转录因子在大豆响应环境胁迫中的作用。基因注释，数据挖掘的压力反应，并从两个家庭的成员的系统发育分析。大豆基因组中至少存在45个bHLH（来自亚组25）和63个trihelix-GT推定基因。其中，至少有14 bHLH和11 trihelix-GT似乎参与响应非生物/生物胁迫。系统发育分析成功地将这些与其他植物物种的成员聚类。然而，bHLH和trihelix-GT基因在大豆中包含的成员几乎是拟南芥或水稻的三倍，其中许多基因在其他分析的物种中没有明显的近直系同源物而分组为新的分支。我们的研究结果是一个重要的一步，解开植物bHLH和trihelix-GT转录因子的功能作用，在响应环境的线索。
Environmental stresses caused by either abiotic or biotic factors greatly affect agriculture. As for soybean [Glycine max (L.) Merril], one of the most important crop species in the world, the situation is not different. In order to deal with these stresses, plants have evolved a variety of sophisticated molecular mechanisms, to which the transcriptional regulation of target-genes by transcription factors is crucial. Even though the involvement of several transcription factor families has been widely reported in stress response, there still is a lot to be uncovered, especially in soybean. Therefore, the objective of this study was to investigate the role of bHLH and trihelix-GT transcription factors in soybean responses to environmental stresses. Gene annotation, data mining for stress response, and phylogenetic analysis of members from both families are presented herein. At least 45 bHLH (from subgroup 25) and 63 trihelix-GT putative genes reside in the soybean genome. Among them, at least 14 bHLH and 11 trihelix-GT seem to be involved in responses to abiotic/biotic stresses. Phylogenetic analysis successfully clustered these with members from other plant species. Nevertheless, bHLH and trihelix-GT genes encompass almost three times more members in soybean than in Arabidopsis or rice, with many of these grouping into new clades with no apparent near orthologs in the other analyzed species. Our results represent an important step towards unraveling the functional roles of plant bHLH and trihelix-GT transcription factors in response to environmental cues.