A holistic approach for selection of Bacillus spp. as a bioremediator for shrimp postlarvae culture
A holistic approach for selection of Bacillus spp. as a bioremediator for shrimp postlarvae culture
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DOI：
10.3906/biy-1203-19
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发表时间：
2013-01-01
影响因子：
2.2
通讯作者：
Khatoon, Helena
中科院分区：
文献类型：
--
作者：
Devaraja, Thimmalapura;Banerjee, Sanjoy;Khatoon, Helena
土著短小芽孢杆菌B. licheniformis）和短小芽孢杆菌（B.从海水和土壤中分离得到枯草芽孢杆菌（subtilis），并研究了其在斑节对虾养殖中的潜在生物修复能力。芽孢杆菌基于它们的宽范围生长条件、易于大量培养、对总氨氮（TAN）的耐受性、对致病弧菌的抑制、非致病性和降低TAN的能力而选择。结果表明，筛选出的芽孢杆菌在不同温度下的最适生长条件为：温度20 ℃，光照时间20min。在30 ℃、pH7.5和1.5%NaCl条件下发生，并分泌蛋白酶、淀粉酶和脂肪酶。弧菌属3种芽孢杆菌也能抑制其生长。此外，所选择的芽孢杆菌属。对虾苗无致病作用，并能降低TAN。在没有水交换的情况下，它们促进了虾PL的更好的生长和存活。本研究是一个系统的方法来选择合适的芽孢杆菌属。作为斑节对虾养殖系统的生物介体。
Indigenous Bacillus pumilus, B. licheniformis, and B. subtilis were isolated from marine water and soil samples and investigated for potential bioremediation ability in Penaeus monodon culture. Bacillus spp. were selected based on their wide range of growth conditions, ease of mass culture, tolerance to total ammonia nitrogen (TAN), inhibition of pathogenic vibrios, nonpathogenicity, and ability to reduce TAN. Results showed that optimum growth of the selected Bacillus spp. occurred at 30 degrees C, pH 7.5, and 1.5% NaCl, and they secreted protease, amylase, and lipase. Vibrio spp. were also inhibited by 3 Bacillus spp. In addition, the selected Bacillus spp. had no pathogenic effect on shrimp postlarvae (PL) and were able to reduce TAN. They promoted better growth and survival in shrimp PL without water exchange. This study was a systematic approach undertaken for the selection of suitable Bacillus spp. as bioremediators for a Penaeus monodon culture system.