Characterization of Transcriptomes of Cochlear Inner and Outer Hair Cells
Characterization of Transcriptomes of Cochlear Inner and Outer Hair Cells
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DOI：
10.1523/jneurosci.1690-14.2014
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发表时间：
2014-08-13
影响因子：
5.3
通讯作者：
He, David Z. Z.
中科院分区：
文献类型：
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作者：
Liu, Huizhan;Pecka, Jason L.;He, David Z. Z.
内毛细胞 (IHC) 和外毛细胞 (OHC) 是两种类型的感觉受体细胞，对哺乳动物耳蜗的听力至关重要。 IHC 和 OHC 具有不同的形态和功能。定义其形态和功能特化的遗传机制基本上是未知的。转录组反映了细胞中活跃表达的基因，是理解细胞生物学特性分子机制的关键。使用 DNA 微阵列，我们检查了从成年小鼠耳蜗中单独收集的 2000 个 IHC 和 OHC 的转录组。我们发现 IHC 和 OHC 中分别表达了 16,647 和 17,711 个转录本。在这些基因中，两个群体中相似的 73% 是已知基因，22% 是未表征的序列，5.0% 是非编码 RNA。两个群体中总共表达了 16,117 个转录本。独特和差异表达的基因解释了
Inner hair cells (IHCs) and outer hair cells (OHCs) are the two types of sensory receptor cells that are critical for hearing in the mammalian cochlea. IHCs and OHCs have different morphology and function. The genetic mechanisms that define their morphological and functional specializations are essentially unknown. The transcriptome reflects the genes that are being actively expressed in a cell and holds the key to understanding the molecular mechanisms of the biological properties of the cell. Using DNA microarray, we examined the transcriptome of 2000 individually collected IHCs and OHCs from adult mouse cochleae. We show that 16,647 and 17,711 transcripts are expressed in IHCs and OHCs, respectively. Of those genes, similar to 73% are known genes, 22% are uncharacterized sequences, and 5.0% are noncoding RNAs in both populations. A total of 16,117 transcripts are expressed in both populations. Uniquely and differentially expressed genes account for