Evaluation of two probiotics used during farm production of white shrimp Litopenaeus vannamei (Crustacea: Decapoda)
Evaluation of two probiotics used during farm production of white shrimp Litopenaeus vannamei (Crustacea: Decapoda)
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凡纳滨对虾（甲壳类：十足目）养殖过程中使用的两种益生菌的评价
DOI：
10.1111/are.13031
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
Nestor M Castillo
中科院分区：
文献类型：
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作者：
Ramón Franco;L. Martín;A. Arenal;Dayamí Santiesteban;J. Sotolongo;H. Cabrera;J. Mejías;George Rodríguez;Angela G Moreno;E. Pimentel;Nestor M Castillo
本研究在两个独立的实验中，检测了从凡纳滨对虾肠道分离出的、对哈维氏弧菌具有拮抗活性的地衣芽孢杆菌CIGBC - 232菌株，在不同个体发育阶段对凡纳滨对虾免疫力和幼体质量的影响：（1）在实验室条件下，从PL2到PL17阶段的幼虾暴露于CIGBC - 232菌株；（2）在养殖条件下，从溞状幼体I期到PL8阶段的幼虾暴露于CIGBC - 232菌株和EPICIN 3W益生菌。第一个实验表明，与未处理的对照组相比，使用CIGBC - 232培养的动物其酚氧化酶、过氧化物酶和超氧化物歧化酶活性均有所提高。在测试中，其对渗透压的抵抗力也有所增强。在第二个实验中，与接受EPICIN 3W的动物相比，使用CIGBC - 232处理的动物酚氧化酶活性显著（P < 0.05）提高（在溞状幼体I - II期、糠虾幼体II - III期和PL2 - 7期提高30 - 40%），呼吸爆发也显著提高（在PL2到7期提高30%）。在凝集素和凝集素原方面（除PL1、6和7外）、过氧化物酶和超氧化物歧化酶活性、对渗透压的抵抗力以及各处理组之间的存活率均无显著差异。与EPICIN 3W处理相比，CIGBC - 232处理能够降低养殖池水体以及动物体内假定弧菌属的水平。在两个实验结束时，CIGBC - 232处理使虾的生长，即体重和体长增加。本研究表明了CIGBC - 232的益生菌效应，其似乎比EPICIN 3W处理具有更好的益生菌性能。
This study examined the effects of Bacillus licheniformis strain CIGBC-232, isolated from the gut of shrimp Litopenaeus vannamei and having antagonistic activity against Vibrio harveyi, on the immunity and larval quality of L. vannamei at various ontogenetic stages, in two separate experiments: (1) PL2 to PL17 exposed to the strain CIGBC-232 under laboratory conditions (2) zoea I to PL8 exposed to the strain CIGBC-232 and EPICIN 3W probiotics under farming conditions. The first experiment showed that phenoloxidase, peroxidase and superoxide dismutase activities were improved in animals grown with CIGBC-232 compared to the untreated control. In tests, the resistance to osmotic stress was also enhanced. During the second experiment, animals treated with CIGBC-232 exhibited significant (P < 0.05) increases in phenoloxidase activity (30–40% higher in zoea I–II, mysis II–III and PL 2–7) and in the respiratory burst (30% higher from PL 2 to 7) as compared to those animals that received EPICIN 3W. There was no significant difference in the lectins and agglutinins (except in PL1, 6 and 7), in peroxidase and superoxide dismutase activities, in the resistance to osmotic stress, nor in the survival rate among treatments. CIGBC-232 treatment was able to reduce, the level of presumptive Vibrio spp. with respect to EPICIN 3W treatment in the tank water as well as in animals. At the end of both experiments, the growth of shrimp, i.e. weight and length was increased by CIGBC-232 treatment. This study showed the probiotic effect of CIGBC-232, which appeared to have a better probiotic performance than EPICIN 3W treatment.