Daily rhythm of glutathione peroxidase activity, lipid peroxidation and glutathione levels in tissues of pinealectomized rats
Daily rhythm of glutathione peroxidase activity, lipid peroxidation and glutathione levels in tissues of pinealectomized rats
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DOI：
10.1016/s0304-3940(02)00144-1
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发表时间：
2002-05-03
影响因子：
2.5
通讯作者：
Canatan, H
中科院分区：
文献类型：
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作者：
Baydas, G;Gursu, MF;Canatan, H
褪黑激素是抗氧化防御系统的组成部分，因为它具有自由基清除和抗氧化活性。在本研究中，我们旨在研究松果体切除大鼠（PINX）组织中抗氧化酶谷胱甘肽过氧化物酶（GSH-Px）活性、氧化型谷胱甘肽（GSSG）和脂质过氧化水平的内源性节律。每隔 4 小时通过断头处死大鼠。 GSH-Px 活性、GSSG 和脂质过氧化水平在对照组和 PINX 大鼠中均显示出每日节律。 GSH-Px 和 GSSG 在其他组先前确定的褪黑激素峰值时间之后表现出峰值水平。脂质过氧化水平在夜间逐渐升高，并在 GSH-Px 峰值时间之前开始下降。这些发现表明，内源性褪黑激素参与夜间 GSH-Px 活性和 GSSG 水平的增加，并调节 GSH-Px 的日节律模式。总之，消除褪黑激素节律的松果体切除术对 GSH-Px 活性水平具有抑制作用。 (C) 2002 Elsevier Science Ireland Ltd. 保留所有权利。
Melatonin is a component of the antioxidant defense system since it has radical scavenging and antioxidant activities. In the present study, we aimed to investigate the endogenous rhythm of antioxidant enzyme glutathione peroxidase (GSH-Px) activity, oxidized glutathione (GSSG) and lipid peroxidation levels in tissues of pinealectomized rats (PINX). Rats were sacrificed by decapitation at 4 h intervals. GSH-Px activity, GSSG and lipid peroxidation levels showed a daily rhythm both in controls and in PINX rats. GSH-Px and GSSG exhibited the peak levels after the peak time of melatonin which was determined previously by other groups. Lipid peroxidation levels increased progressively during the night and started to decline before the GSH-Px peak time. These findings suggest that endogenous melatonin is involved in the night time increase of GSH-Px activity and GSSG levels and modulates the daily rhythm pattern of GSH-Px. In conclusion, pinealectomy which eliminates the melatonin rhythm has a supressor effect on GSH-Px activity levels. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.