Comparison of the multiple oligomeric structures observed for the Rvb1 and Rvb2 proteins
Comparison of the multiple oligomeric structures observed for the Rvb1 and Rvb2 proteins
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DOI：
10.1139/o09-159
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发表时间：
2010-02-01
期刊：
影响因子：
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通讯作者：
Ortega, Joaquin
中科院分区：
文献类型：
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作者：
Cheung, Kevin L. Y.;Huen, Jennifer;Ortega, Joaquin
Rvb 1和Rvb 2蛋白是AAA+家族的2个成员，参与染色质重塑、转录、小核仁RNA成熟、细胞转化、细胞凋亡和有丝分裂的信号传导等多种作用。这些蛋白质能够在如此多样的细胞活动中发挥作用，因为它们是不同大分子组装体的组分。在过去的几年中，已经有许多小组报告了纯化的Rvbs的结构。报道的结果一直是相当有争议的，因为有显着的差异，尽管这些蛋白质之间的高度同源性，但在已发表的结构之间观察到。令人惊讶的是，不仅在代表来自不同物种的Rvb蛋白的结构之间观察到矛盾，而且在来自相同物种的蛋白质结构之间也观察到矛盾。本文综述了不同物种中Rvb的结构，并对它们进行了比较分析。最后，我们确定了这些结构研究的一些方面值得进行额外的调查，以确保报告的结构反映生理相关的构象的RVB 1-RVB 2复合物。
The Rvb1 and Rvb2 proteins are 2 members of the AAA+ family, involved in roles as diverse as chromatin remodeling, transcription, small nucleolar RNA maturation, cellular transformation, signaling of apoptosis and mitosis. These proteins are capable of playing a role in such diverse cellular activities because they are components of different macromolecular assemblies. In the last few years, there has been a number of groups reporting on the structure of purified Rvbs. The reported results have been rather controversial, because there are significant differences observed among the published structures in spite of the high degree of homology among these proteins. Surprisingly, contradictions are observed not only between structures representing the Rvb proteins from different species, but also between protein structures from the same species. This review describes the available Rvb structures from different species and also makes a comparative analysis of them. Finally, we identify some aspects of these structural studies worth pursuing in additional investigations to ensure that the reported structures reflect physiologically relevant conformations of the Rvb1–Rvb2 complex.