Slow and fast muscle fibers are preferentially derived from myoblasts migrating into the chick limb bud at different developmental times.
Slow and fast muscle fibers are preferentially derived from myoblasts migrating into the chick limb bud at different developmental times.
复制标题
慢肌纤维和快肌纤维优先源自在不同发育时间迁移到雏鸡肢芽中的成肌细胞。
DOI：
10.1006/dbio.1995.1218
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Lance-Jones,C
中科院分区：
文献类型：
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作者：
VanSwearingen,J;Lance-Jones,C
鸟类肢体成肌细胞起源于体节，并在肢体芽形成期间迁移到外周。目前尚不清楚这些前体是如何排列成肌肉和初级纤维类型的定型模式的。我们使用体内手术移植和大腿肌肉模式的解剖分析来询问在不同发育时间迁移到肢芽的成肌细胞是否采用不同的命运。当成肌细胞迁移中断移植肢芽组织体腔的宿主胚胎早期在迁移期（阶段16-早期17），很少大腿肌肉被发现在阶段30-33。存在的原基对应于通常含有大部分慢肌管的肌肉。在稍晚移植的肢体中（晚期17-18），唯一缺失的肌肉是那些通常含有最多快速肌管的肌肉。在迁移期间的不同时间将鹌鹑肢芽移植到鸡宿主中制成的嵌合肢中获得了平行的结果，该实验情况下肢不耗尽肌肉前体或神经。这些结果表明，最早的成肌细胞迁移主要产生慢初级肌管，后来迁移到快肌管。为了确定早期肢芽环境是否定义了迁移成肌细胞的命运，我们评估了从年轻肢芽组织（15- 16早期）移植到老年宿主（17期）的肢体中的纤维类型模式。在慢肌内发现慢肌球蛋白阳性分布的显著耗尽。快肌的大小一般正常。这些结果提供了体内证据表明，肢体成肌细胞的多样性出现之前，成肌细胞进入肢体。我们认为，有一个逐渐变化的成肌细胞的比例能够形成慢和快的纤维类型，这种变化可能开始在体节或早期的迁移期。
Avian limb myoblasts originate from somites and migrate into the periphery during limb bud formation. It is not known how these precursors become arranged into a stereotyped pattern of muscles and primary fiber types. We used in vivo surgical transplantation and anatomical analyses of thigh muscle patterns to ask whether myoblasts migrating into the limb bud at different developmental times adopt different fates. When myoblast migration was interrupted by transplanting limb bud tissue to the coelomic cavity of a host embryo early in the migratory period (stages 16-early 17), few thigh muscles were found at stages 30-33. Primordia that were present corresponded to muscles that normally contain a majority of slow myotubes. In limbs transplanted slightly later (stages late 17-18), the only missing muscles were those that normally contain the highest numbers of fast myotubes. Parallel results were obtained in chimeric limbs made by transplanting a quail limb bud to a chick host at different times during the migratory period, an experimental situation in which the limbs were not depleted of muscle precursors or nerves. These findings suggest that the earliest myoblast migrants give rise mainly to slow primary myotubes, the later migrants to fast myotubes. To determine whether the early limb bud environment defines the fate of migrating myoblasts, we assessed fiber type patterns in limbs that developed from young limb bud tissue (stages 15-early 16) transplanted to older hosts (stage 17). A significant depletion of slow myosin-positive profiles was found within slow muscles. Fast muscles were generally normal in size. These results provide in vivo evidence that limb myoblast diversity arises prior to the entry of myoblasts into the limb. We suggest that there is a gradual change in the proportions of myoblasts capable of forming slow and fast fiber types, a change which may begin in the somites or early in the migratory period.