Protocol for generation and regeneration of PEG-passivated slides for single-molecule measurements.
Protocol for generation and regeneration of PEG-passivated slides for single-molecule measurements.
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用于单分子测量的PEG钝化载玻片的生成和再生方案。
DOI：
10.1016/j.xpro.2022.101152
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发表时间：
2022-03-18
期刊：
影响因子：
--
通讯作者：
Myong S
中科院分区：
文献类型：
--
作者：
Paul T;Myong S
用全内反射显微镜进行单分子荧光检测需要聚乙二醇膜进行表面钝化，这是一项劳动强度很大的工作，而且只能进行一两次实验。在这里，我们提出了一种高效可靠的协议，用于在同一通道中进行多轮实验(∼5-10次)来生成和重新生成聚乙二醇面。该协议非常简单、健壮、快速和通用；即，可以实施多种策略来再生不同的表面层。该再生策略节省了时间，提高了成本效益，提高了单分子实验的效率。有关使用和执行此配置文件的完整详细信息，请参阅。聚乙二醇钝化玻片的再生简单、快速、经济高效可在单一通道内进行多次实验针对不同再生水平实施不同的再生策略再生导致高度重复性结果全内反射显微镜检测单分子荧光需要聚乙二醇膜表面钝化，这是劳动密集型的，只能用于一到两个实验。在这里，我们提出了一种高效可靠的协议，用于在同一通道中进行多轮实验(∼5-10次)来生成和重新生成聚乙二醇面。该协议非常简单、健壮、快速和通用；即，可以实施多种策略来再生不同的表面层。该再生策略节省了时间，提高了成本效益，提高了单分子实验的效率。
Single-molecule fluorescence detection by total internal reflection microscope requires surface passivation by polyethylene glycol (PEG) coating, which is labor intensive and is only good for one or two experiments. Here, we present an efficient and reliable protocol for generating and regenerating the PEG surface for multiple rounds of experiments (∼5–10 times) in the same channel. This protocol is very simple, robust, rapid, and versatile; i.e., multiple strategies can be implemented to regenerate different layers of surface. The regeneration strategy saves time, improves the cost effectiveness, and enhances the efficiency of single-molecule experiments. For complete details on the use and execution of this profile, please refer to. Regeneration of PEG-passivated slide is simple, quick, and cost effective Multiple experiments can be performed in a single channel Different strategies are implemented for different level of regeneration Regeneration leads to highly reproducible results Single-molecule fluorescence detection by total internal reflection microscope requires surface passivation by polyethylene glycol (PEG) coating, which is labor intensive and is only good for one or two experiments. Here, we present an efficient and reliable protocol for generating and regenerating the PEG surface for multiple rounds of experiments (∼5–10 times) in the same channel. This protocol is very simple, robust, rapid, and versatile; i.e., multiple strategies can be implemented to regenerate different layers of surface. The regeneration strategy saves time, improves the cost effectiveness, and enhances the efficiency of single-molecule experiments.
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