PROGESTIN RECEPTOR-CONTAINING CELLS IN GUINEA-PIG HYPOTHALAMUS AFFERENT CONNECTIONS MORPHOLOGICAL CHARACTERISTICS AND NEUROTRANSMITTER CONTENT
PROGESTIN RECEPTOR-CONTAINING CELLS IN GUINEA-PIG HYPOTHALAMUS AFFERENT CONNECTIONS MORPHOLOGICAL CHARACTERISTICS AND NEUROTRANSMITTER CONTENT
复制标题
DOI：
10.1016/1044-7431(90)90042-3
复制
发表时间：
1990-01-01
影响因子：
3.5
通讯作者：
NAFTOLIN F
中科院分区：
文献类型：
--
作者：
BROWN T J;MACLUSKY N J;NAFTOLIN F
通过免疫细胞化学方法检查豚鼠下丘脑中含有孕激素受体的细胞，以表征其形态特性、传入连接和神经递质含量。通过使用针对兔子宫孕激素受体的单克隆小鼠抗体，在雌激素治疗后，在视交叉后区、内侧和脑室周围视前区、弓状核和下丘脑外侧区（包括腹内侧核的腹外侧部分）鉴定出含有孕激素受体的细胞。超微结构分析揭示了仅限于细胞核的异质分布的免疫反应产物。在子宫内膜中观察到类似的核孕激素受体免疫染色模式。黄体酮的施用增强了孕激素受体的免疫染色，但似乎没有改变免疫反应产物在细胞核内的分布。在来自卵巢切除 (OVX) 或 OVX 孕酮处理的动物的切片中未观察到免疫染色。对雌激素/孕激素处理的豚鼠的下丘脑切片进行孕激素受体和神经递质或神经肽相关抗原的双重免疫染色。在孕激素受体免疫阳性神经元上发现了酪氨酸羟化酶、谷氨酸脱羧酶（GAD）、血清素、促肾上腺皮质激素和胆囊收缩素免疫反应性轴突末端的突触接触。此外，在 TH 免疫反应性细胞体和孕激素受体免疫反应性神经元细胞体之间观察到非常规连接复合物。在孕激素受体免疫反应性神经元中，没有观察到除 GAD 之外的神经递质或神经肽免疫染色的证据。除了豚鼠下丘脑中孕激素受体免疫反应性的超微结构特征之外，这些研究还首次证明了参与神经内分泌调节的神经递质/神经肽系统与啮齿动物下丘脑中含有类固醇受体的神经元的直接联系。下丘脑中的雌激素/孕激素敏感细胞可能作为整合激素和神经影响生殖功能控制的中心。
Progestin receptor-containing cells in the guinea pig hypothalamus were examined by immunocytochemical methods to characterize their morphological properties, afferent connections, and neurotransmitter content. Through the use of a monoclonal mouse antibody raised against the rabbit uterine progestin receptor, progrestin receptor-containing cells were identified after estrogen treatment in the retrochiasmatic area, medial and periventricular preoptic area, arcuate nucleus, and lateral hypothalamic area including the ventrolateral aspect of the ventromedial nucleus. Ultrastructural analysis revealed a heterogeneously distributed immunoreaction product restricted to the cell nucleus. A similar pattern of nuclear progestin receptor immunostaining was observed in the uterine endometrium. Progesterone administration intensified progestin receptor immunostaining, without appearing to alter the distribution of immunoreaction product within the cell nucleus. No immunostaining was observed in sections from ovariectomized (OVX) or OVX progesterone-treated animals. Hypothalamic sections from estrogen/progesterone-treated guinea pigs were double-immunostained for progestin receptor and neurotransmitter- or neuropeptide-related antigens. Synaptic contacts of tryosine hydroxylase-, glutamic acid decarboxylase (GAD)-, sertonin-, adrenocorticotropic hormone-, and cholecystokinin-immunoreactive axon terminals were found on progestin receptor-immunopositive neurons. In addition, nonconventional junctional complexes were observed between TH-immunoreactive soma and the soma of progestin receptor-immunoreactive neurons. No evidence of immunostaining for neurotransmitters or neuropeptides other than GAD was observed in progestin receptor-immunoreactive neurons. In addition to ultrastructural characterization of progestin receptor immunoreactivity in the guinea pig hypothalamus, these studies demonstrate, for the first time, direct connections of neurotransmitter/neuropeptide systems involved in neuroendocrine regulation with steroid receptor-containing neurons in the rodent hypothalamus. Estrogen/progesterone-sensitive cells in the hypothalamus may act as centers for the integration of hormonal and neural influences on the control of reproductive function.