Levels of Ethyl Glucuronide and Ethyl Sulfate in Oral Fluid, Blood, and Urine After Use of Mouthwash and Ingestion of Nonalcoholic Wine
Levels of Ethyl Glucuronide and Ethyl Sulfate in Oral Fluid, Blood, and Urine After Use of Mouthwash and Ingestion of Nonalcoholic Wine
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DOI：
10.1093/jat/34.2.84
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发表时间：
2010-03-01
影响因子：
2.5
通讯作者：
Christophersen, Asbjorg
中科院分区：
文献类型：
--
作者：
Hoiseth, Gudrun;Yttredal, Borghild;Christophersen, Asbjorg
本研究的目的是研究大量使用漱口水和摄入无酒精葡萄酒（已证明含有3 mg/L EtG、1.5 mg/L EtS和0.2 g/L乙醇）后，口腔液中乙基葡糖苷酸（EtG）以及血液和尿液中EtG和硫酸乙酯（EtS）的浓度。12名受试者参加了对照实验。所有饮用非酒精性葡萄酒的受试者的尿液样本均显示EtG阴性，但EtS阳性（Cmax 2.15 mg/L）。所有使用漱口水的四名受试者的尿液中EtG和EtS均为阴性。所有口腔液样本均为EtG阴性，所有血液样本均为EtG和EtS阴性。这项研究表明，摄入EtG和EtS作为非酒精葡萄酒的成分，导致检测尿液中的EtS，这表明口服摄入EtS的生物利用度优于EtG。当使用EtG和EtS作为酒精复发标志物时，必须记住摄入非酒精葡萄酒后尿液中EtS假阳性结果的可能性。最后，研究表明，意外酒精暴露后，血液和口腔液中的EtG或EtS结果不太可能呈阳性。
The aim of this study is to investigate the concentrations of ethyl glucuronide (EtG) in oral fluid and both EtG and ethyl sulfate (EtS) in blood and urine following intense use of mouthwash and ingestion of nonalcoholic wine, which are proven to contain 3 mg/L EtG, 1.5 mg/L EtS, and 0.2 g/L ethanol. Twelve subjects participated in a controlled experiment. All subjects ingesting nonalcoholic wine showed urine samples negative for EtG but positive for EtS (Cmax 2.15 mg/L). All four subjects using mouthwash were negative for EtG and EtS in urine. All samples of oral fluid were negative for EtG and all samples of blood were negative for EtG and EtS. This study showed that ingestion of EtG and EtS as components of nonalcoholic wine lead to detection of urine EtS only, suggesting superior bioavailability of orally ingested EtS compared to EtG. This possibility of false-positive EtS results in urine after ingestion of nonalcoholic wine is important to remember when using EtG and EtS as relapse markers for alcohol. Finally, the study showed that a positive EtG or EtS result after accidental alcohol exposure is unlikely in blood and oral fluid.