Reverse micelle-loaded lipid nano-emulsions: New technology for nano-encapsulation of hydrophilic materials
Reverse micelle-loaded lipid nano-emulsions: New technology for nano-encapsulation of hydrophilic materials
复制标题
DOI：
10.1016/j.ijpharm.2010.07.039
复制
发表时间：
2010-10-15
影响因子：
5.8
通讯作者：
Saulnier, Patrick
中科院分区：
文献类型：
--
作者：
Anton, Nicolas;Mojzisova, Halina;Saulnier, Patrick
这项研究提出了一种新的、最近获得专利的技术，用于将亲水性物质封装在脂质纳米乳液中。该方法基于相转变温度法（即所谓的 PIT 法），遵循低能量且无溶剂的过程。形成的纳米乳液可稳定数月，液滴尺寸范围为 10 至 200 nm。荧光素钠盐的亲水模型分子通过在反胶束系统中的溶解被封装在这些纳米乳液液滴的油核中。结果，产生了具有“水包油反胶束”结构中的亲水分子的原始多尺度纳米物体。一旦荧光素被封装，由于热力学原因，它保持稳定，并且封装率可以达到 90%。之所以能够形成如此复杂的物体，是由于使用了软方法（PIT方法），该方法允许在整个配制过程中保持反胶束的结构，直到它们被捕获在纳米乳液液滴中。在本研究中，我们将调查重点放在过程本身上，揭示其潜力和局限性。由于用于封装亲水物质的纳米载体的配方仍然是一个挑战，这项研究可能构成该领域的重大进展。 (C) 2010 Elsevier B.V. 保留所有权利。
This study presents novel, recently patented technology for encapsulating hydrophilic species in lipid nano-emulsions. The method is based on the phase-inversion temperature method (the so-called PIT method), which follows a low-energy and solvent-free process. The nano-emulsions formed are stable for months, and exhibit droplet sizes ranging from 10 to 200 nm. Hydrophilic model molecules of fluorescein sodium salt are encapsulated in the oily core of these nano-emulsion droplets through their solubilisation in the reverse micellar system. As a result, original, multi-scaled nano-objects are generated with a 'hydrophilic molecule in a reverse-micelles-in-oil-in-water' structure. Once fluorescein has been encapsulated it remains stable, for thermodynamic reasons, and the encapsulation yields can reach 90%. The reason why such complex objects can be formed is due to the soft method used (PIT method) which allows the conservation of the structure of the reverse micelles throughout the formulation process, up to their entrapment in the nano-emulsion droplets. In this study, we focus the investigation on the process itself, revealing its potential and limits. Since the formulation of nanocarriers for the encapsulation of hydrophilic substances still remains a challenge, this study may constitute a significant advance in this field. (C) 2010 Elsevier B.V. All rights reserved.