Analysis of Single Chain Antibody Sequences Using the VBASE2 Fab Analysis Tool
Analysis of Single Chain Antibody Sequences Using the VBASE2 Fab Analysis Tool
复制标题
使用 VBASE2 Fab 分析工具分析单链抗体序列
DOI：
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发表时间：
2010
期刊：
影响因子：
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通讯作者：
Werner Müller
中科院分区：
文献类型：
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作者：
S. Mollova;Ida Retter;M. Hust;S. Dübel;Werner Müller
先前描述了VBASE 2数据库的生成（Retter等人，2005）。VBASE 2数据库目前包含1类人61个重链可变基因片段、50个κ轻链可变基因片段和49个λ轻链可变基因片段。对于小鼠，数据库保存了153个重链可变基因片段、77个κ轻链可变基因片段和3个λ 1类轻链序列。数据库的完整统计数据可以在网站菜单的V基因统计部分下访问（图1.1）。从互联网页面，用户可以在VBASE2数据库中进行查询，并通过简单地点击统计表中的引用编号来查看一个类别的所有基因条目。用户还可以在网站菜单的下载部分下载VBASE 2数据库中包含的V基因序列。VBASE2数据库中存在的每个V基因片段具有唯一的标识号。在这个数字后面，数据库中存在单个V基因片段条目，其提供给定基因的关键信息。图1.2显示了这样一个条目的例子。这是1类序列的一个例子，表明生殖系基因和重排都是已知的。功能已显示。列出了所有已知的序列名称。给出了V基因家族，并提供了最后更新的日期。核苷酸和蛋白质序列都以FASTA格式显示（并且可以很容易地复制以进行进一步分析和存储）。关键特征的位置
The generation of the VBASE2 database was previously described (Retter et al. 2005). The VBASE2 database currently holds for the human 61 heavy chain variable gene segments, 50 kappa light chain variable gene segments and 49 lambda light chain variable gene segments of Class 1. For the mouse, the database keeps 153 heavy chain variable gene segments, 77 kappa light chain variable gene segments and three lambda light chain sequences of Class 1. The complete statistics of the database can be accessed under the V Gene Statistics section of the website menu (Fig. 1.1). From the Internet page, the user can make a query in the VBASE2 database and view all gene entries of a class by simply clicking on the referring number in the statistics table. The user can also download the V gene sequences contained in the VBASE2 database under theDownload section of the website menu. Each V gene segment present in the VBASE2 database has a unique identification number. Behind this number, an individual V gene segment entry is present in the database, which provides key information of a given gene. An example of such an entry is shown in Fig. 1.2. This is an example of a Class 1 sequence indicating that both the germline gene and the rearrangements are known. The functionality is indicated. All known sequence names are presented. The V gene family is given and the date of the last update is provided. Both the nucleotide and the protein sequences are shown in FASTA format (and can be easily copied for further analyses and storage). The position of key features