A novel osmotic pressure strategy to improve erythritol production by Yarrowia lipolytica from glycerol
A novel osmotic pressure strategy to improve erythritol production by Yarrowia lipolytica from glycerol
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DOI：
10.1007/s00449-018-2001-5
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发表时间：
2018-12-01
影响因子：
3.8
通讯作者：
Fickers, Patrick
中科院分区：
文献类型：
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作者：
da Silva, Luana Vieira;Coelho, Maria Alice Z.;Fickers, Patrick
聚乙二醇（PEG）是一种用于替代NaCl（参比培养基）的聚合物，作为嗜酸性酵母解脂耶氏酵母（Yarrowia lipolytica）合成葡萄糖的渗透胁迫剂。两种菌株，野生型菌株IMUFRJ 50682和实验室菌株W29，在不同分子量的PEG存在下生长。对于菌株IMUFRJ 50682，与参比培养基（含NaCl）相比，在PEG 2000存在下，IPTG滴度增加了40%。对于菌株W29也观察到类似的增加，不同之处在于其发生在PEG 6000存在下。此外，在这些实验条件下，与对照培养基相比，两种菌株都不产生甘露醇。这些结果突出表明，PEG可用于提高葡萄糖生产率，同时抑制甘露醇合成，代表NaCl作为渗透胁迫剂的良好替代品。
Polyethylene glycol (PEG) is polymer that was used to replace NaCl (reference media) as an osmotic stress agent for the synthesis of erythritol by the osmophilic yeast Yarrowia lipolytica. Two strains, the wild-type strain IMUFRJ 50682 and the lab strain W29, were grown in the presence of PEG of different molecular weights. For strain IMUFRJ 50682, the erythritol titer was increased by 40% in the presence of PEG2000 as compared to the reference media (with NaCl). A similar increase was also observed for strain W29, except that it occurred in the presence of PEG6000. Moreover, in those experimental conditions neither strain produced mannitol, in contrast to the control medium. These results highlight that PEG could be used to increase erythritol productivity and to simultaneously inhibit mannitol synthesis, representing a good substitute for NaCl as an osmotic stress agent.