Preparation and structural determination of large oligosaccharides derived from acharan sulfate
Preparation and structural determination of large oligosaccharides derived from acharan sulfate
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DOI：
10.1016/j.carres.2006.02.030
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发表时间：
2006-05-22
影响因子：
3.1
通讯作者：
Linhardt, RJ
中科院分区：
文献类型：
--
作者：
Chi, L;Munoz, EM;Linhardt, RJ
对一系列由硫酸阿查烷衍生的大寡糖进行了结构表征。Acharan sulfate是一种从非洲大蜗牛Achatina fulica中分离出来的糖胺聚糖。使用肝素裂解酶11酶促制备从十糖到六-十糖的寡糖并纯化。毛细管电泳和凝胶电泳证实了这些寡糖的纯度。其结构经ESI-MS和NMR确证，与硫酸阿查烷中的主要重复序列一致。-> 4）-a-D-GlcN（p）Ac-（1 -> 4）-a-L-IdoA（p）2S-（1 ->，在还原端被4-连接的a-D-GlcN（p）Ac残基封端，在非还原端被4，5-不饱和吡喃糖基糖醛酸2-硫酸酯封端。(c)2006爱思唯尔有限公司保留所有权利。
The structures of a series of large oligosaccharides derived from acharan sulfate were characterized. Acharan sulfate is an unusual glycosaminoglycan isolated from the giant African snail, Achatina fulica. Oligosaccharides from decasaccharide to hexa-decasaccharide were enzymatically prepared using heparin lyase 11 and purified. Capillary electrophoresis and gel electrophoresis confirmed the purity of these oligosaccharides. Their structures, determined by ESI-MS and NMR, were consistent with the major repeating sequence in acharan sulfate. -> 4)-alpha-D-GlcN(p)Ac-(1 -> 4)-alpha-L-IdoA(p)2S-(1 ->, terminated by 4-linked alpha-D-GlcN(p)Ac residue at the reducing end and by 4,5-unsaturated pyranosyluronic acid 2-sulfate at the non-reducing end. (c) 2006 Elsevier Ltd. All rights reserved.