Kindling alters the calcium/calmodulin-dependent phosphorylation of synaptic plasma membrane proteins in rat hippocampus.
Kindling alters the calcium/calmodulin-dependent phosphorylation of synaptic plasma membrane proteins in rat hippocampus.
复制标题
Kindling 改变大鼠海马突触质膜蛋白的钙/钙调蛋白依赖性磷酸化。
DOI：
10.1073/pnas.81.4.1253
复制
发表时间：
1984
影响因子：
11.1
通讯作者：
D. Farber
中科院分区：
文献类型：
--
作者：
C. Wasterlain;D. Farber
隔核点燃与抑制大鼠海马区几种突触质膜蛋白的HOC后磷酸化有关。在对照大鼠中，结合钙/钙调素显著刺激~(32)P掺入分子量为50,000、58,000和60,000的蛋白质，而在点燃动物中，对结合钙/钙调素的反应减弱。钙离子、cAMP或cGMP单独调节~(32)P掺入几种突触质膜蛋白，但不能区分对照和点燃组织。在促肾上腺皮质激素和[亮氨酸]脑啡肽的HOC后实验中，对照组和点燃大鼠均表现出对钙/钙调素刺激的磷酸化的非特异性抑制。对照组和点燃动物之间的差异在海马区和杏仁内嗅区最显著；在皮质、基底节和脑干不明显；在小脑不明显，因为小脑是无法引发点燃的区域。点燃后8周的休息期并没有减少这些变化，这表明它们可能是一种持续的点燃行为本身。
Septal kindling was associated with an inhibition of the post hoc phosphorylation of several synaptic plasma membrane proteins of rat hippocampus. In control rats, the 32P incorporation into proteins of molecular weights 50,000, 58,000, and 60,000 was markedly stimulated by combined calcium/calmodulin, whereas in kindled animals, the response to combined calcium/calmodulin was reduced. Calcium alone, cAMP, or cGMP modulated 32P incorporation into several synaptic plasma membrane proteins but did not differentiate control from kindled tissues. Both control and kindled rats showed nonspecific inhibition of calcium/calmodulin-stimulated phosphorylation in the post hoc assay by corticotropin and by [Leu]enkephalin. The differences between control and kindled animals were most striking in hippocampus and in the amygdaloid-entorhinal area; less pronounced in cortex, basal ganglia, and brain stem; and not significant in cerebellum, a region where kindling cannot be elicited. An 8-wk period of rest after kindling did not reduce these changes, suggesting that they may be a persistent as the kindling behavior itself.