Expression and distribution of junctional adhesion molecule-1 in the human cornea
Expression and distribution of junctional adhesion molecule-1 in the human cornea
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DOI：
10.1007/s10384-007-0479-5
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发表时间：
2007-11-01
影响因子：
2.4
通讯作者：
Murakami, Akira
中科院分区：
文献类型：
--
作者：
Chen, Lizhong;Ebihara, Nobuyuki;Murakami, Akira
Purpose: To investigate the expression and distribution of junctional adhesion molecule 1 (JAM-1) in human corneal tissue and cells.Methods: Reverse transcriptase-polymerase chain reaction was used to detect the expression of JAM-1, ZO-1, and occludin mRNAs in corneal cells, while the presence of JAM-1 protein was analyzed by flow cytometry (FACS). Double immunofluorescence staining was used to determine the tissue distribution of JAM-1 and occludin in human corneas.Results: Strong expression of JAM-1, ZO-1, and occludin mRNAs was observed in primary cultured corneal epithelial and endothelial cells, but not in primary cultured keratocytes. The expression of JAM-1 protein in cultured epithelial and endothelial cells was confirmed by FACS. When keratocytes were cultured in medium with 10% fetal calf serum for several passages, differentiation into corneal myofibroblasts occurred. The expression of JAM-1 was detected in these corneal myofibroblasts at both RNA and protein levels. JAM-1 immunoreactivity was seen at cell borders throughout the entire epithelium, but not in keratocytes from normal corneal tissue. On the other hand, JAM-1 immunoreactivity was detected in the cytoplasm of corneal endothelial cells. Jpn J Ophthalmol 2007;51: 405-411 (c) Japanese Ophthalmological Society 2007Conclusions: JAM-1 is expressed by human corneal epithelial and endothelial cells, but not by keratocytes, although its expression is induced in corneal myofibroblasts.
