Overexpression of a mitochondrial ATP synthase small subunit gene (AtMtATP6) confers tolerance to several abiotic stresses in Saccharomyces cerevisiae and Arabidopsis thaliana
Overexpression of a mitochondrial ATP synthase small subunit gene (AtMtATP6) confers tolerance to several abiotic stresses in Saccharomyces cerevisiae and Arabidopsis thaliana
复制标题
DOI：
10.1007/s10529-008-9685-6
复制
发表时间：
2008-03
影响因子：
2.7
通讯作者：
Xinxin Zhang;Shenkui Liu;T. Takano
中科院分区：
文献类型：
--
作者：
Xinxin Zhang;Shenkui Liu;T. Takano
线粒体F1 F0-ATP酶是植物代谢中的关键酶，为细胞提供ATP，利用跨膜电化学质子梯度驱动ATP的合成。从拟南芥线粒体F1 F0-ATP酶中分离到一个分子量为6 kDa的蛋白At 3g 46430。在这项研究中，基因（AtMtATP 6; GenBank登录号AK 117680）编码这种蛋白质的分离和鉴定。北方杂交分析表明，盐、干旱和低温等非生物胁迫诱导了拟南芥AtMtATP 6基因的表达。AtMtATP 6基因在转基因酵母和拟南芥中的过表达增强了对盐、干旱、氧化和低温胁迫的抗性。综上所述，我们的数据提出了诱导F1 F0-ATP酶在胁迫耐受性中起作用的可能性。
Mitochondrial F1F0-ATPase is a key enzyme in plant metabolism, providing cells with ATP that uses the transmembrane electrochemical proton gradient to drive synthesis of ATP. A 6 kDa protein (At3g46430) has been previously purified fromArabidopsis thalianamitochondrial F1F0-ATPase. In this study, the gene (AtMtATP6; GenBank accession no. AK117680) encoding this protein was isolated fromArabidopsisand characterized. Northern blot analyses showed that the expression ofAtMtATP6gene inArabidopsissuspension-cultured cells was induced by several abiotic stresses from salts, drought, and cold. Over-expression ofAtMtATP6gene in transgenic yeast andArabidopsisplants increased the resistance to salts, drought, oxidative and cold stresses. Taken together, our data raise the possibility that induction of the F1F0-ATPase plays a role in stress tolerance.