Distribution of NADPH-diaphorase and nitric oxide synthase (NOS) in different regions of porcine oviduct during the estrous cycle
Distribution of NADPH-diaphorase and nitric oxide synthase (NOS) in different regions of porcine oviduct during the estrous cycle
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DOI：
10.1177/002215540004800615
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发表时间：
2000-06-01
影响因子：
3.2
通讯作者：
Ziecik, AJ
中科院分区：
文献类型：
--
作者：
Gawronska, B;Bodek, G;Ziecik, AJ
一氧化氮合酶（NOS）负责多个器官（包括生殖道器官）中一氧化氮（NO）的生物产生。我们研究了猪输卵管中NADPH-黄递酶（NADPH-d）活性（NOS活性标记物）的潜在变化以及NOS的存在和分布。在发情周期的不同日期从后备母猪（n=16）中获得组织。一条输卵管用于组织学和免疫组织化学，另一条用于Western印迹分析。输卵管粘膜上皮NADPH-d活性明显高于输卵管肌层。NADPH-d活性最高的部位是峡部上皮。峡部上皮中的反应强度（任意单位+/- SEM）从排卵后期开始增加，直至发情前期（96.2 +/- 11.2），然后逐渐降低。峡部上皮NADPH-d反应强度在发情期最低（58.4 +/- 7.7）。在发情周期的排卵后阶段观察到输卵管所有部分的输卵管肌中NADPH-d活性最强（峡部38.3 +/- 2.5;壶腹35.6 +/- 4.2;漏斗部24.7 +/- 0.8），然后在发情周期的其余阶段下降（p< 0.001）。在发情周期的所有研究日，在粘膜上皮细胞和血管组织和输卵管肌的内皮细胞中检测到内皮型一氧化氮合酶（eNOS）的存在。诱导型一氧化氮合酶（inducibleNOS，iNOS）阳性反应仅限于淋巴管和部分血管内皮。由于我们的蛋白质印迹分析表明，猪输卵管含有eNOS，但没有iNOS，我们认为，eNOS是在猪输卵管中表达的NOS的主要亚型。我们认为，输卵管不同部位NADPH-d活性的不同，以及在发情周期中其活性的变化，可能表明NO在调节输卵管功能中的重要作用。
Nitric oxide synthase (NOS) is responsible for the biological production of nitric oxide (NO) in several organs, including those of the reproductive tract. We investigated potential changes in NADPH-diaphorase (NADPH-d) activity (marker for NOS activity) and the presence and distribution of NOS in the porcine oviduct. Tissues were obtained from gilts (n=16) on different days of the estrous cycle. One fallopian tube was used for histo-and immunohistochemistry and the other for Western blotting analysis. NADPH-d activity was much higher in the epithelium of the mucosa than in the myosalpinx. The highest activity of NADPH-d was always found in the epithelium of the isthmus. The intensity of the reaction (arbitrary units +/- SEM) in isthmus epithelium increased from the postovulatory period until early proestrus (96.2 +/- 11.2) and then gradually decreased. The lowest intensity of NADPH-d reaction in the epithelium of the isthmus was seen at estrus (58.4 +/- 7.7). The most intense NADPH-d activity in myosalpinx of all parts of the oviduct was observed at the postovulatory stage of the estrous cycle (isthmus 38.3 +/- 2.5; ampulla 35.6 +/- 4.2; infundibulum 24.7 +/- 0.8) and then decreased during the remaining stages of the estrous cycle (p< 0.001). The presence of endothelial NOS (eNOS) was detected in epithelial cells of mucosa and in endothelium of vascular tissues and myosalpinx during all studied days of the estrous cycle. The positive reaction for inducible NOS (iNOS) was restricted only to the endothelium of lymph vessels and some blood vessels. Because our Western blotting analysis revealed that porcine oviduct contains eNOS but not iNOS, we suggest that eNOS is the main isoform of NOS expressed in the porcine oviduct. We concluded that the different activity of NADPH-d in the various regions of the oviduct, accompanied by changes in its activity during the course of the estrous cycle, could indicate an important role of NO in regulation Of tubal function.