Immunolocalization of a candidate pheromone receptor in the antenna of the male moth, Heliothis virescens
Immunolocalization of a candidate pheromone receptor in the antenna of the male moth, Heliothis virescens
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DOI：
10.1007/s10158-005-0012-9
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发表时间：
2006-03-01
影响因子：
--
通讯作者：
Krieger, Juergen
中科院分区：
文献类型：
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作者：
Gohl, Thomas;Krieger, Juergen
信息素在昆虫中的识别被认为涉及触角感觉神经元树突膜上的不同受体蛋白。我们已经产生了针对来自烟芽夜蛾的候选信息素受体HR13的序列的肽的抗体。这些抗体特异性地标记了位于雄性特异性信息素敏感感器中的不同神经元群体的细胞体。结合抗体染色和原位杂交，发现反应细胞表达HR 13基因。此外，树突投射到感器的头发，以及免疫反应细胞的轴突过程进行了标记。轴突的标记已经允许它们在触角段内的成束可视化，并且允许在轴突合并到触角神经中时跟踪轴突。在羽化前1天首次检测到HR13蛋白。因此，HR13蛋白在雄蛾触角神经元中的分布强烈表明HR13受体在识别信息素中的作用。
Pheromone recognition in insects is thought to involve distinct receptor proteins in the dendritic membrane of antennal sensory neurons. We have generated antibodies directed against a peptide derived from the sequence of the candidate pheromone receptor HR13 from Heliothis virescens. The antibodies specifically labelled the cell bodies of a distinct neuron population housed in male-specific pheromone-sensitive sensilla. Combining antibody staining with in situ hybridization the reactive cells were found to express the HR13 gene. In addition, dendrites projecting into sensilla hairs as well as the axonal processes of immunoreactive cells were labelled. Labelling of axons has allowed visualization of their fasciculation within antennal segments and permits tracking of axons as they merge into the antennal nerve. The HR13 protein was first detected 1 day before eclosion. Thus, the distribution of HR13 protein in the antennal neurons of the male moth strongly suggests a role of the HR13 receptor in recognition of pheromones.