Genetic control of root plasticity in response to salt stress in maize
Genetic control of root plasticity in response to salt stress in maize
复制标题
玉米根系可塑性响应盐胁迫的遗传控制
DOI：
10.1007/s00122-021-03784-4
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发表时间：
2021-03-04
影响因子：
5.4
通讯作者：
Yang, Zefeng
中科院分区：
文献类型：
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作者：
Li, Pengcheng;Yang, Xiaoyi;Yang, Zefeng
关键信息Gwas确定了559个与响应盐的根构型重塑相关的显著SNPs，并通过整合RNA-seq、DEG和WGCNA数据对168个候选基因进行了优先排序。盐度是限制作物生长和生产力的主要环境因素。根是第一个遇到盐胁迫的植物器官，但盐分对玉米根发育的影响尚不清楚。本研究对319个玉米自交系在对照和盐分条件下的14个根部和4个地上部性状进行了自然变异分析。所有性状都有较大的表型变异，高盐浓度降低了根长，但增加了根径。对这些性状及其可塑性(相对变异)进行了全基因组关联研究。检测到559个显著的单核苷酸多态，其中125个、181个和253个分别与对照条件、应激条件和性状可塑性相关。在全基因组关联研究确定的587个候选基因中，共有168个得到差异表达基因或共表达网络的支持。对两个候选基因ZmIAA1和ZmGRAS43进行了重测序验证。在这些基因中，有130个是在胁迫条件或性状可塑性条件下检测到的，这些基因参与了植物激素信号转导、苯丙烷生物合成和脂肪酸生物合成等多种生物学过程。我们的发现阐明了根对盐分的改造，所识别的基因座和候选基因可能对玉米根系性状的遗传改良和耐盐性具有重要意义。
Key message GWAS identified 559 significant SNPs associated with the remodelling of the root architecture in response to salt, and 168 candidate genes were prioritized by integrating RNA-seq, DEG and WGCNA data. Salinity is a major environmental factor limiting crop growth and productivity. The root is the first plant organ to encounter salt stress, yet the effects of salinity on maize root development remain unclear. In this study, the natural variations in 14 root and 4 shoot traits were evaluated in 319 maize inbred lines under control and saline conditions. Considerable phenotypic variations were observed for all traits, with high salt concentrations decreasing the root length, but increasing the root diameter. A genome-wide association study was conducted to analyse these traits and their plasticity (relative variation). We detected 559 significant single nucleotide polymorphisms, of which 125, 181 and 253 were associated with the control condition, stress condition and trait plasticity, respectively. A total of 168 of 587 candidate genes identified by genome-wide association study were supported by the differentially expressed genes or co-expression networks. Two candidate genes ZmIAA1 and ZmGRAS43 were validated by resequencing. Among these genes, 130 were detected under stress condition or trait plasticity that involved in diverse biological processes including plant hormone signal transduction, phenylpropanoid biosynthesis and fatty acid biosynthesis. Our findings clarify the root remodelling to salinity, and the identified loci and candidate genes may be important for the genetic improvement of root traits and salt tolerance in maize.