Sodium and Potassium Interactions with Nucleic Acids
Sodium and Potassium Interactions with Nucleic Acids
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DOI：
10.1007/978-3-319-21756-7_6
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发表时间：
2016-01-01
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影响因子：
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通讯作者：
Ennifar, Eric
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文献类型：
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作者：
Auffinger, Pascal;D'Ascenzo, Luigi;Ennifar, Eric
金属离子是核酸结构和功能的重要辅助因子。然而，早在70年代就发现了镁离子在稳定tRNA结构中的关键作用，这一发现在很长一段时间内掩盖了单价阳离子的重要性。90年代末，在I族内含子核心中发现了特定的钾金属离子，重新引起了人们对这些离子的兴趣。它们在核酸折叠和催化活性中的重要性现在已经得到了充分的证实。然而，K+和Na+离子的检测是出了名的问题，关于它们的特异性的问题反复出现。本文综述了用于检测核酸结构中K+和Na+离子的不同方法，如X射线结晶学、核磁共振或分子动力学模拟。我们还通过不同的例子讨论了不同结构的特定结合和非特定结合。
Metal ions are essential cofactors for the structure and functions of nucleic acids. Yet, the early discovery in the 70s of the crucial role of Mg2+ in stabilizing tRNA structures has occulted for a long time the importance of monovalent cations. Renewed interest in these ions was brought in the late 90s by the discovery of specific potassium metal ions in the core of a group I intron. Their importance in nucleic acid folding and catalytic activity is now well established. However, detection of K+ and Na+ ions is notoriously problematic and the question about their specificity is recurrent. Here we review the different methods that can be used to detect K+ and Na+ ions in nucleic acid structures such as X-ray crystallography, nuclear magnetic resonance or molecular dynamics simulations. We also discuss specific versus non-specific binding to different structures through various examples.