Association of A beta 40-positive senile plaques with microglial cells in the brains of patients with Alzheimer's disease and in non-demented aged individuals
Association of A beta 40-positive senile plaques with microglial cells in the brains of patients with Alzheimer's disease and in non-demented aged individuals
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DOI：
10.1006/neur.1996.0002
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发表时间：
1996-03-01
期刊：
影响因子：
--
通讯作者：
Iwatsubo, T
中科院分区：
文献类型：
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作者：
Fukumoto, H;AsamiOdaka, A;Iwatsubo, T
为了深入了解小胶质细胞在老年斑（SP）形成中的作用，特别是在产生具有不同羧基（C）端的淀粉样β蛋白（A β）的两种主要分子种类A β 40和A β 42中的作用（43），我们对阿尔茨海默病（AD）和非阿尔茨海默病患者的脑组织切片进行了双重免疫标记研究，使用针对A β的C-端蛋白和铁蛋白（小胶质细胞的标志物）的抗体对痴呆老年个体进行研究。AD和非痴呆个体中所有SP均为A β 42（43）阳性，其中只有一部分为A β 40阳性。在AD和非痴呆个体中，大约2/3的A β 40阳性SP是具有淀粉样蛋白核心的典型SP，这些几乎总是与小胶质细胞相关。A β 40阳性的未核心SP也经常与小胶质细胞相关（平均74%），而A β 40阴性的未核心SP中只有24%含有小胶质细胞。这些结果表明，小胶质细胞可能在SP的成熟中发挥作用，特别是在A β 40的产生中。(C)出版社：Academic Press Limited。
To gain insight into the role of microglia in the formation of senile plaques (SP), especially in the generation of the two major molecular species of amyloid beta protein (A beta) with different carboxyl (C)-tenmini, A beta 40 and A beta 42(43), we conducted double immunolabelling studies on tissue sections from the brains of Alzheimer's disease (AD) and non-demented aged individuals using antibodies to the C-ternini of A beta and ferritin, a marker for microglia. All SP were A beta 42(43)positive in AD as well as in non-demented individuals, only a proportion of which were A beta 40-positive. Both in AD and in non-demented individuals, approximately 2/3 of the A beta 40-positive SP were typical SP with amyloid cores, these being almost invariably associated with microglia. A beta 40-positive, uncored SP were also frequently associated with microglia (mean, 74%), whereas only 24% of A beta 40-negative, uncored SP contained microglia. These results suggest that microglia may play a role in the maturation of SP, especially in the generation of A beta 40. (C) 1996 Academic Press Limited.