INVOLVEMENT OF SUPEROXIDE ANION GENERATION IN THE HYPERSENSITIVE RESPONSE OF POTATO-TUBER TISSUES TO INFECTION WITH AN INCOMPATIBLE RACE OF PHYTOPHTHORA-INFESTANS AND TO THE HYPHAL WALL COMPONENTS
INVOLVEMENT OF SUPEROXIDE ANION GENERATION IN THE HYPERSENSITIVE RESPONSE OF POTATO-TUBER TISSUES TO INFECTION WITH AN INCOMPATIBLE RACE OF PHYTOPHTHORA-INFESTANS AND TO THE HYPHAL WALL COMPONENTS
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DOI：
10.1016/0048-4059(83)90019-x
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
DOKE, N
中科院分区：
文献类型：
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作者：
DOKE, N
马铃薯块茎组织光盘，这是年龄受伤后，以获得过敏反应，减少细胞外细胞色素c和硝基蓝四唑（NBT）接种后，与不兼容，但不兼容，比赛的致病疫霉。细胞色素C还原活性迅速增加，从接种后1至4小时沿着的超敏死亡细胞的百分比增加，然后下降的时间时，大多数穿透细胞已经死亡。一个本地化的激活NBT减少入侵菌丝周围的不兼容，但不是那些兼容的，种族观察到在渗透的早期阶段，细胞死亡之前。的还原活性的光盘也引起了治疗与过敏引起的物质，菌丝壁成分的真菌。超氧化物歧化酶（SOD）是一种催化超氧阴离子（O2-）转化为H2 O2的酶，当添加到测定溶液中时，O2抑制了圆盘还原活性的增强，这表明细胞色素c和NBT可能被圆盘产生的O2-还原。感染前，真空渗透的光盘与含有SOD的溶液显着延迟过敏性细胞死亡的发生所造成的感染与不兼容的种族，以及积累的植物抗毒素。SH结合试剂和NADP+的应用，但不是呼吸抑制剂，抑制诱导的还原活性所造成的感染与不兼容的种族。一个O2-产生系统可能被激活的马铃薯组织中的不相容的相互作用过程中引起的入侵真菌或真菌壁成分; O2-的产生可能涉及过敏性细胞死亡作为一个触发器的抗性反应的序列。
Potato tuber tissue discs, which were aged after wounding in order to acquire hypersensitive reactivity, reduced extracellular cytochrome c and nitroblue tetrazolium (NBT) following inoculation with an incompatible, but not compatible, race of P. infestans. The cytochrome-C-reducing activity rapidly increased from 1 to 4 h after inoculation along with an increase in the percentage of hypersensitively dead cells, and then decreased from the time when most of the penetrated cells had died. A localized activation of NBT reduction around invading hyphae of the incompatible, but not those of the compatible, race was observed at early stages of penetration before cell death. The reductive activity of the discs was also elicited by treatment with a hypersensitivity-eliciting substance, hypal wall components of the fungus. Superoxide dismutase (SOD), an enzyme catalysing the conversion of the superoxide anion (O2-) to H2O2 and O2 inhibited the enhanced reducing activity of the discs when added to the assay solution, indicating that cytochrome c and NBT may be reduced by O2- generated from the discs. Pre-infectional, vacuum infiltration of the discs with a solution containing SOD significantly delayed the occurrence of hypersensitive cell death caused by infection with the incompatible race as well as the accumulation of phytoalexin. Application of SH-binding reagents and NADP+, but not respiratory inhibitors, inhibited the elicitation of the reducing activity caused by infection with the incompatible race. An O2-generating system may be activated in potato tissues during the incompatible interaction induced by invading fungi or fungal wall components; the generation of O2- may be involved during hypersensitive cell death as a trigger of the sequence of resistance reactions.