Overexpression of gma-miR1510a/b suppresses the expression of a NB-LRR domain gene and reduces resistance to Phytophthora sojae
Overexpression of gma-miR1510a/b suppresses the expression of a NB-LRR domain gene and reduces resistance to Phytophthora sojae
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DOI：
10.1016/j.gene.2017.04.015
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发表时间：
2017-07-20
期刊：
影响因子：
3.5
通讯作者：
Xing, Han
中科院分区：
文献类型：
--
作者：
Cui, Xiaoxia;Yan, Qiang;Xing, Han
microRNAs（miRNAs）是真核生物中普遍存在的抑制靶基因表达的调控因子，在植物免疫应答中起重要作用。最近发现miRNAs参与大豆和大豆疫霉的相互作用。在此，我们筛选了miR 1510，该miR 1510在大豆疫霉感染期间在大豆中被抑制，表明其可能参与大豆对病原体的应答。为了进一步揭示miRNAs在大豆中的作用，以拟南芥miR 319 a前体为骨架，在大豆毛状根中过表达grna-miR 1510 a/B。gma-miR 1510 a/b过表达的毛状根表现出对大豆疫霉的敏感性增强，并且结果表明miR 1510指导Glyma.16G135500基因的切割，该基因编码一种经典类型的植物抗病相关基因，该基因含有Toll-白介素样受体（TIR）结构域和核苷酸结合位点-富含亮氨酸重复序列（NB-LRR）结构域。值得注意的是，在gma-MIR 1510 a和目的基因的启动子中发现了几种生物胁迫和胁迫响应的顺式调控元件。总的来说，本研究中获得的结果表明，gmamiR 1510调节靶NB-LRR免疫受体基因Glyma.16G135500，因此在调节大豆对大豆疫霉的抗性中起关键作用。
MicroRNAs (miRNAs) are universal regulators that repress target gene expression in eukaryotes and play essential roles in plant immune responses. miRNAs were recently found to be involved in soybean and Phytophthora sojae interactions. Here, we screened miR1510, which was repressed in soybean during infection with P. sojae, indicating that it might be involved in soybean response to pathogens. To further uncover the roles of miRNAs in soybean, grna-miR1510a/b was overexpressed in the hairy roots of soybean using an Arabidopsis miR319a precursor as the backbone. The gma-miR1510a/b-overexpressing hairy roots showed enhanced susceptibility to P. sojae, and the results showed that miR1510 guides the cleavage of the Glyma.16G135500 gene, which encodes a classic type of plant disease resistance-associated gene that harbors the Toll-interleukin-like receptor (TIR) domain and nucleotide-binding site-leucine-rich repeat (NB-LRR) domain. Noticeably, several biotic stresses and hormone-responsive cis-regulatory elements were found to be present in the promoters of gma-MIR1510a and the target gene. Collectively, the results obtained in the current study reveal that gmamiR1510 regulates the target NB-LRR immune receptor gene Glyma.16G135500 and thus plays a crucial role in regulating the resistance of soybean to P. sojae.