The long non-coding RNA CRNDE promotes cervical cancer cell growth and metastasis
The long non-coding RNA CRNDE promotes cervical cancer cell growth and metastasis
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DOI：
10.1515/hsz-2017-0199
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发表时间：
2018-01-01
影响因子：
3.7
通讯作者：
Ma, Rong
中科院分区：
文献类型：
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作者：
Meng, Yuanyuan;Li, Qi;Ma, Rong
本研究旨在分析lncRNA CRNDE对宫颈癌细胞生长和转移的影响。收集50对宫颈癌组织及相应的癌旁组织。通过微阵列分析检测组织样本中长非编码RNA (lncRNA) 的表达。采用qRT-PCR检测宫颈癌细胞和正常细胞中CRNDE的表达水平。利用细胞计数试剂盒-8 (CCK-8) 测定和克隆形成测定来评估细胞生长。采用伤口愈合实验和Transwell实验检测宫颈癌细胞的迁移和侵袭能力。 CRNDE在宫颈癌组织和细胞中的表达高于正常组织和细胞。 CCK-8实验和克隆形成实验表明，敲低CRNDE可以抑制HeLa和C-33A细胞的细胞增殖。伤口愈合实验表明CRNDE表达下调可以抑制细胞迁移。 Transwell实验结果表明，与Mock组相比，CRNDE-si组的侵袭细胞数量减少。 LncRNA CRNDE可以促进细胞生长并刺激宫颈癌细胞的转移。
This study was intended to analyze effects of lncRNA CRNDE on cervical cancer cell growth and metastasis. Fifty pairs of cervical cancer tissues and corresponding adjacent tissues were collected. Expressions of long non-coding RNAs (lncRNAs) in tissue samples were detected by microarray analysis. Expression levels of CRNDE in cervical cancer cells and normal cells were detected by qRT-PCR. Cell-counting kit-8 (CCK-8) assay and clone formation assay were utilized to evaluate cell growth. Wound healing assay and Transwell assay were conducted to detect the migratory and invasive capability of cervical cancer cells. The expressions of CRNDE in cervical cancer tissues and cells were higher than those in normal tissues and cells. CCK-8 assay and clone formation assay showed that the knockdown of CRNDE could inhibit the cell proliferation of HeLa and C-33A cells. Wound healing assay indicated that the downregulation of CRNDE expression could suppress the cell migration. The result of a Transwell assay demonstrated that the number of invasion cells reduced in the CRNDE-si group in comparison with the Mock group. LncRNA CRNDE could promote the cell growth and stimulate the metastasis of cervical cancer cells.