MALDI Mass Spectrometry Imaging for the Simultaneous Location of Resveratrol, Pterostilbene and Viniferins on Grapevine Leaves
MALDI Mass Spectrometry Imaging for the Simultaneous Location of Resveratrol, Pterostilbene and Viniferins on Grapevine Leaves
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DOI：
10.3390/molecules190710587
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发表时间：
2014-07-01
期刊：
影响因子：
4.6
通讯作者：
Chaimbault, Patrick
中科院分区：
文献类型：
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作者：
Becker, Loic;Carre, Vincent;Chaimbault, Patrick
为了研究对胁迫的原位响应，对葡萄树叶进行了质谱成像（MSI）实验。使用不同基质的基质辅助激光解吸/电离 (MALDI) 方法已得到评估。在所有测试的基质中，2,5-二羟基苯甲酸 (DHB) 被发现是最有效的基质，可以检测到更广泛的二苯乙烯植物抗毒素。鉴定并绘制了白藜芦醇以及对真菌更具毒性的化合物，例如紫檀芪和葡萄素。它们在紫外线照射下的葡萄叶子上的空间分布显示了它们在叶脉周围的特定排列。此外，MALDI MSI 显示，当叶子被葡萄生单轴霉感染时，白藜芦醇（和云杉类）和葡萄素并不特异地位于同一区域。 MALDI 质谱成像获得的结果表明，该技术对于提高有关二苯乙烯植物抗毒素在应激事件中的作用的知识水平至关重要。
To investigate the in-situ response to a stress, grapevine leaves have been subjected to mass spectrometry imaging (MSI) experiments. The Matrix Assisted Laser Desorption/Ionisation (MALDI) approach using different matrices has been evaluated. Among all the tested matrices, the 2,5-dihydroxybenzoic acid (DHB) was found to be the most efficient matrix allowing a broader range of detected stilbene phytoalexins. Resveratrol, but also more toxic compounds against fungi such as pterostilbene and viniferins, were identified and mapped. Their spatial distributions on grapevine leaves irradiated by UV show their specific colocation around the veins. Moreover, MALDI MSI reveals that resveratrol (and piceids) and viniferins are not specifically located on the same area when leaves are infected by Plasmopara viticola. Results obtained by MALDI mass spectrometry imaging demonstrate that this technique would be essential to improve the level of knowledge concerning the role of the stilbene phytoalexins involved in a stress event.