GEOGRAPHIC ORIGIN OF THE Y-CHROMOSOMES IN OLD INBRED STRAINS OF MICE
GEOGRAPHIC ORIGIN OF THE Y-CHROMOSOMES IN OLD INBRED STRAINS OF MICE
复制标题
DOI：
10.1007/bf00292153
复制
发表时间：
1992-01-01
期刊：
影响因子：
2.5
通讯作者：
EICHER, EM
中科院分区：
文献类型：
--
作者：
TUCKER, PK;LEE, BK;EICHER, EM
用5个探针从39个标准近交系小鼠中鉴定了6个不同的Y染色体(Chr)，在Southern blotts上鉴定了Y Chr特异的限制性片段。31个菌株中有3种Y-Chr亚型，均起源于亚洲小鼠。其余3个YChr类型分布于8个菌株中，均起源于家蝇。通过测定三个近交系(C57BL/6J、AKR/J和SWR/J)Sry(性别决定区Y)基因(自然346：245-250,1990)中221个碱基的DNA序列，并将该序列与来自欧洲和亚洲野生捕获的肌肉支原体雄性的同源序列进行比较，证实了YChr的亚洲来源。这些数据表明，至少有6只雄性小鼠促成了旧近交系的形成。
Six distinct Y Chromosomes (Chr) were identified among 39 standard inbred strains of mice with five probes that identified Y Chr-specific restriction fragments on Southern blots. Three Y Chr types, distributed among 31 strains, were of Asian Mus musculus origin. The remaining three Y Chr types, distributed among eight strains, were of M. domesticus origin. The Asian source of the M. musculus Y Chr was confirmed by determining the DNA sequence of 221 bp from an open reading frame within the Sry (sex determining region Y) gene (Gubbay et al., Nature 346: 245-250, 1990) in three inbred strains (C57BL/6J, AKR/J, and SWR/J) and comparing the sequence to the homologous sequences derived from wild caught European and Asian M. musculus males. These data indicate that a minimum of six male mice contributed to the formation of the old inbred strains.