Site specific recombination: Developments and applications
Site specific recombination: Developments and applications
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DOI：
10.1016/0958-1669(94)90068-x
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发表时间：
1994-01-01
影响因子：
7.7
通讯作者：
Sauer, Brian
中科院分区：
文献类型：
--
作者：
Sauer, Brian
位点特异性DNA重组系统在操纵DNA方面具有相当大的实用性，并且可以促进许多克隆和基因转移技术。许多重组酶在高等真核生物中催化有效DNA重组的能力对于精确的染色体和转基因工程具有重要和令人兴奋的结果。这些重组酶的利用将促进转基因动物疾病模型的产生，并有助于阐明发育基因的功能。
Site-specific DNA recombination systems have considerable utility in manipulating DNA and can facilitate many cloning and gene transfer techniques. The ability of a number of recombinases to catalyze efficient DNA recombination in higher eukaryotes has important and exciting consequences for precise chromosome and transgene engineering. Exploitation of these recombinases will facilitate the generation of transgenic animal disease models and help elucidate the function of developmental genes.