EFFECTS OF HIGH ELECTRIC FIELDS ON MICRO-ORGANISMS .3. LYSIS OF ERYTHROCYTES AND PROTOPLASTS
EFFECTS OF HIGH ELECTRIC FIELDS ON MICRO-ORGANISMS .3. LYSIS OF ERYTHROCYTES AND PROTOPLASTS
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DOI：
10.1016/0005-2736(68)90030-8
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发表时间：
1968-01-01
期刊：
影响因子：
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通讯作者：
HAMILTON, WA
中科院分区：
文献类型：
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作者：
SALE, AJH;HAMILTON, WA
本文研究了高压电场对细菌原生质体、球体和红细胞的裂解作用。将高达25千伏/厘米的电场作为一系列直流脉冲施加到细胞悬浮液中；填充的溶解程度取决于磁场强度。计算了球状细胞跨膜的电势差；当该电势差达到约1V(根据物种的值从0.7到1.15V)时，发生裂解。结果表明，该电势可能引起膜结构的构象变化，导致膜的半透性能丧失。
The lysis of bacterial protoplasts, spheroplasts and erythrocytes by high electric fields has been examined. Fields up to 25 kV/cm were applied as a series of direct current pulses to suspensions of the cells; the degree of lysis of a populatoin depended upon the field strength. The potential difference across the membrane of the spherical cell has been calculated; lysis occurred when this potential difference reached about 1 V (values ranged from 0.7 to 1.15 V according to the species). It is suggested that the potential may cause conformational changes in the membrane structure resulting in the observed loss of its semipermeable properties.